GenMute™ siRNA Transfection Reagent

- Overview
  - GenMute™ Reagent is a novel biodegradable non-liposomal siRNA/miRNA & DNA delivery tool. With our proprietary pH Dependent Conformational Change ( PDCC) technology, the biodegradable transfection reagent was formulated by addition of pre-screened hydrophobic groups to its side chain, making GenMute™ Reagent a versatile and most powerful gene delivery tool in the market. GenMute™ Reagent have been validated to effectively and reproducibly transfect single siRNA/miRNA or co-transfect DNA/siRNA or DNA/miRNA to variety of mammalian cells.
    
    - Excellent silencing at low concentrations with only 1.0 nM siRNA / miRNA mimics
    - Minimizing non-specific and off target effects in the cell
    - One tube reaction
    - Best for broad mammalian cells
    - Very low cytotoxicity
    - Very affordable
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    In Vitro siRNA Transfection Reagents
    
    Other Properties
    
    Content:
    -GenMute™ Reagent, 1.0 mL, sufficient for ~2, 000 reactions based on transfecting 5.0 pmol siRNA or miRNA mimics in 24-well plate.
    -GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 mL to make 40 mL working solution.
    
    Storage
    
    Store at 4 °C for GenMute™ Reagent and RT for GenMute™ Transfection Buffer (5x ). If stored properly, the product is stable for 12 months or longer.
    
    Note
    
    PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium. Culturing your cells with other cell media (supplemented with 10% FBS and antibiotics) will basically eliminate the transfection toxicity. If you find toxicity and high cell death, it may be because DMEM base medium supplemented with 10% FBS and antibiotics was used to culture the cells. To remove the transfection toxicity, DMEM can be replaced with DMEM/F12 or RPMI 1640 as base medium. Although we haven't figured out why, it does minimize toxicity. Please try it!
    
    * This product is for laboratory research ONLY and not for diagnostic use.
- Applications
  - Application Description
    
    - Single siRNA transfection or DNA/siRNA co-transfection to variety of mammalian cells.
    - Single miRNA, miRNA mimics or inhibitors transfection or DNA/miRNA or DNA/miRNA mimics co-transfection to variety of mammalian cells.

Documents

A General Protocol for Transfecting siRNA to Mammalian Cells

A General Protocol for Transfecting Mammalian Cells

GenMute™ siRNA Transfection Reagent

GenMute™ siRNA Transfection Reagent

Documents

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