GenMute™ siRNA Transfection Reagent for U2OS Cell

- Overview
  - GenMute™ siRNA Transfection Reagent for U2OS Cell is pre-optimized for transfecting siRNA to U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the bone tissue of a fifteen-year-old human female suffering from osteosarcoma. Established in 1964, the original cells were taken from a moderately differentiated sarcoma of the tibia. U2OS cells exhibit epithelial adherent morphology and viruses were not detected in the line during co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and insulin-like growth factor II (IGF II) receptors and express a number of antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Pre-optimized Transfection Reagents
    
    Other Properties
    
    Reporter Gene: Renilla Luciferase
    Content:
    -GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 15 pmoles siRNA or miRNA mimics in 24-well plate
    -GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
    
    Storage
    
    Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
    
    Note
    
    PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium. Culturing your cells with other cell media (supplemented with 10% FBS and antibiotics) will basically eliminate the transfection toxicity. If you find toxicity and high cell death, it may be because DMEM base medium supplemented with 10% FBS and antibiotics was used to culture the cells. To remove the transfection toxicity, DMEM can be replaced with DMEM/F12 or RPMI 1640 as base medium. Although we haven't figured out why, it does minimize toxicity. Please try it!
    
    * This product is for laboratory research ONLY and not for diagnostic use.

Documents

Data Sheet & Protocol

GenMute™ siRNA Transfection Reagent for U2OS Cell

GenMute™ siRNA Transfection Reagent for U2OS Cell

Documents

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