GenMute™ siRNA Transfection Reagent for MCF7 Cell

- Overview
  - GenMute™ siRNA Transfection Reagent for MCF7 Cell is pre-optimized for transfecting siRNA to MCF7 cells. MCF-7 cell is a breast cancer cell line was isolated in 1970 from a 69-year-old Caucasian woman. This cell line retained several characteristics of differentiated mammary epithelium including the ability to process estradiol via cytoplasmic estrogen receptors and the capability of forming domes. This cell line expresses the oncogene Wnt7B. Tumor necrosis factor alpha (TNF alpha) inhibits the growth of MCF-7 breast cancer cells. Treatment with anti-estrogens can modulate the secretion of insulin-like growth factor binding protein's.
    
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- Properties
  - Categories
    
    Pre-optimized Transfection Reagents
    
    Other Properties
    
    Reporter Gene: Renilla Luciferase
    Content:
    -GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles siRNA or miRNA mimics in 24-well plate
    -GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
    
    Storage
    
    Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
    
    Note
    
    PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium. Culturing your cells with other cell media (supplemented with 10% FBS and antibiotics) will basically eliminate the transfection toxicity. If you find toxicity and high cell death, it may be because DMEM base medium supplemented with 10% FBS and antibiotics was used to culture the cells. To remove the transfection toxicity, DMEM can be replaced with DMEM/F12 or RPMI 1640 as base medium. Although we haven't figured out why, it does minimize toxicity. Please try it!
    
    * This product is for laboratory research ONLY and not for diagnostic use.

Documents

Data Sheet & Protocol

GenMute™ siRNA Transfection Reagent for MCF7 Cell

GenMute™ siRNA Transfection Reagent for MCF7 Cell

Documents

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