GenMute™ siRNA Transfection Reagent for Jurkat Cell

- Overview
  - GenMute™ siRNA Transfection Reagent for Jurkat Cell i s pre-optimized for transfecting siRNA to Jurkat cells. J urkat cells are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Pre-optimized Transfection Reagents
    
    Other Properties
    
    Reporter Gene: Renilla Luciferase
    Content:
    -GenMute™ Reagent, 1.0 ml, sufficient for ~833 reactions based on transfecting 10 pmoles siRNA or miRNA mimics in 24-well plate
    -GenMute™ Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml to make 40 ml working solution
    
    Storage
    
    Store at 4 °C. If stored properly, the product is stable for 12 months or longer.
    
    Note
    
    PepMute™, GenMute™ and PepMute™ Plus siRNA transfection reagents are found to be sometimes toxic ONLY with DMEM base cell culture medium. Culturing your cells with other cell media (supplemented with 10% FBS and antibiotics) will basically eliminate the transfection toxicity. If you find toxicity and high cell death, it may be because DMEM base medium supplemented with 10% FBS and antibiotics was used to culture the cells. To remove the transfection toxicity, DMEM can be replaced with DMEM/F12 or RPMI 1640 as base medium. Although we haven't figured out why, it does minimize toxicity. Please try it!
    
    * This product is for laboratory research ONLY and not for diagnostic use.

Documents

Data Sheet & Protocol

GenMute™ siRNA Transfection Reagent for Jurkat Cell

GenMute™ siRNA Transfection Reagent for Jurkat Cell

Documents

We Also Recommend

GenJet™ In Vitro siRNA Transfection Reagent (Ver. II)

GenJet™ Plus DNA In Vivo Transfection Reagent