GenJet™ In Vitro DNA Transfection Reagent for MDA-MB231 Cells

GenJet™ DNA In Vitro Transfection Reagent for MB231 is pre-optimized for transfecting MDA-MB231 cells. The MDA-MB-231 breast cancer cell line was obtained from a pleural effusion of a 51-year-old white female in October 17, 1973, by Dr. R. Calleau at M. D. Anderson Cancer Center. The patient underwent a mastectomy and radiotherapy prior to the removal of the breast cancer specimen. After 52 passages, the karyotype displayed chromosome numbers ranging between 52 and 62, where the modal chromosome number was 57. With epithelial-like morphology, the MDA-MB-231 breast cancer cells appear phenotypically as spindle shaped cells. In vitro, the MDA-MB-231 cell line has an invasive phenotype. It has abundant activity in both the Boyden chamber chemoinvasion and chemotaxis assay. The MDA-MB-231 cell line is also able to grow on agarose, an indicator of transformation and tumorigenicity, and displays a relatively high colony forming efficiency.

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Background

Tips for selecting diluents for GenJet™ DNA transfection reagents.
For efficient formation of transfection complexes, it is essential to choose an appropriate diluent to dilute the DNA transfection reagent and DNA. In addition to temperature and incubation time, the nature of the diluent is very important for the complete formation of the transfection complex, thus significantly affecting the DNA transfection efficiency. According to our validation data, the appropriate diluent is 50 times more efficient than the "wrong" diluent.

Follow the following technical tips to choose the proper diluent for GenJet™ DNA transfection reagents:
1. For the complete formation of transfection complex, the diluent must be serum-free and protein free.
2. Try to use a diluent that is closest to the composition of cell culture medium. If you are using DMEM (supplemented with serum and antibiotics) to grow HEK293 cells, the best diluent is serum-free and antibiotics-free DMEM. If you are using RPMI-1640 Medium (supplemented with serum and antibiotics) to grow Hela cells, the best diluent is serum/antibiotic-free RPMI-1640 medium.
3. Do not use media containing unknown components. For example, never use Opti-MEM™ to dilute GenJet™ DNA transfection reagents. According to our validation data, diluting our transfection reagents and DNA with Opti-MEM™ at least sacrificed 50% transfection efficiency on HEK293, Hela, CHO and NIH 3T3 cells. In addition, Opti-MEM™ may contains low level of serum, which would greatly compromise transfection efficiency.
4. Basically serum-free DMEM with high glucose is pretty good choice. It is very easily accessible and compatible with other cell growth mediums. If you have trouble with lower transfection efficiency, you may think about changing diluent to serum-free DMEM with high glucose. If you do not know how to choose a proper diluent, you can try serum-free DMEM with high glucose first which usually gives you very good transfection efficiency.