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Overview
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Product Uses Include:
To study the effects of pharmaceutical compounds on the ratio of G-actin to F-actin.
To study the effects of mutated cell lines versus their parent cell line for the change in ratio of G-actin to F-actin.
To study the effects of physical alterations of environment on the ratio of G-actin to F-actin.
Introduction
The most reproducible and accurate method of determining the amount of filamentous actin (F-actin) content versus free globular-actin (G-actin) content in a cell population is to use Western blot quantitation of F-actin and G-actin cellular fractions (1-4). The general approach is to homogenize cells in F-actin stabilization buffer, followed by centrifugation to separate the F-actin from G-actin pool. The fractions are then separated by SDS-PAGE and actin is quantitated by Western blot. The final result gives the most accurate method of determining the ratio of F-actin incorporated into the cytoskeleton versus the G-actin found in the cytosol. This kit contains all the reagents needed to perform this assay.Please contact us at for specific academic pricing.
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Properties
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CategoriesActin Products
* For Research Use Only
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Reference
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Wang, Caiqin et al. Induction of filopodia formation by α-Actinin-2 via RelA with a feedforward activation loop promoting overt bone marrow metastasis of gastric cancer. Journal of Translational Medicine 2023 21:1. 2023
Correa, Francisco et al. Actin-Cytoskeleton Drives Caveolae Signaling to Mitochondria during Postconditioning. Cells. 2023
Bogucka-Janczi, Katarzyna et al. ERK3/MAPK6 dictates CDC42/RAC1 activity and ARP2/3-dependent actin polymerization. eLife. 2023
He, Zheng Quan et al. Pharmacological regulation of tissue fibrosis by targeting the mechanical contraction of myofibroblasts. Fundamental Research. 2022
Lohraseb, Iman et al. Global ubiquitinome profiling identifies NEDD4 as a regulator of Profilin 1 and actin remodelling in neural crest cells. Nature Communications 2022 13:1. 2022
Fang, Shi et al. RhoBTB1 reverses established arterial stiffness in angiotensin II–induced hypertension by promoting actin depolymerization. JCI Insight. 2022
Si, Hongjiang et al. A Highlights from MBoC Selection: Noncanonical Wnt/Ror2 signaling regulates cell–matrix adhesion to prompt directional tumor cell invasion in breast cancer. Molecular Biology of the Cell. 2022
Flores-Muñoz, Carolina et al. The Long-Term Pannexin 1 Ablation Produces Structural and Functional Modifications in Hippocampal Neurons. Cells. 2022
Zhang, Yan et al. Different changes in pre- and postsynaptic components in the hippocampal CA1 subfield after transient global cerebral ischemia. Brain Structure and Function. 2022
Guerrero-Valero, Marta et al. Dysregulation of myelin synthesis and actomyosin function underlies aberrant myelin in CMT4B1 neuropathy. Proceedings of the National Academy of Sciences of the United States of America. 2021
Misra, Suniti et al. Periostin/Filamin-A: A Candidate Central Regulatory Axis for Valve Fibrogenesis and Matrix Compaction. Frontiers in Cell and Developmental Biology. 2021
Landry, Natalie M. et al. SKI activates the Hippo pathway via LIMD1 to inhibit cardiac fibroblast activation. Basic Research in Cardiology. 2021
Kim, Ji Eun et al. PLPP/CIN-mediated NF2-serine 10 dephosphorylation regulates F-actin stability and Mdm2 degradation in an activity-dependent manner. Cell Death and Disease. 2021
Liu, Zhenan et al. Control of Podocyte and Glomerular Capillary Wall Structure and Elasticity by WNK1 Kinase. Frontiers in Cell and Developmental Biology. 2021
Gupta, Richa et al. Ectopic expression of 35 kDa and knocking down of 78 kDa SG2NAs induce cytoskeletal reorganization, alter membrane sialylation, and modulate the markers of EMT. Molecular and cellular biochemistry. 2021
Zhang, Chong et al. Fibrinogen/AKT/Microfilament Axis Promotes Colitis by Enhancing Vascular Permeability. Cellular and molecular gastroenterology and hepatology. 2021
Balbi, Carolina et al. An exosomal-carried short periostin isoform induces cardiomyocyte proliferation. Theranostics. 2021
Saal, Kim Ann et al. Rho-kinase inhibition by fasudil modulates pre-synaptic vesicle dynamics. Journal of Neurochemistry. 2021
Morandell, Jasmin et al. Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development. Nature Communications. 2021
Garcia, Alix et al. miR-204-5p and Platelet Function Regulation: Insight into a Mechanism Mediated by CDC42 and GPIIbIIIa. Thrombosis and haemostasis. 2021
Musah, Aminat S. et al. Mechanistic target of rapamycin regulates the oligodendrocyte cytoskeleton during myelination. Journal of Neuroscience. 2020
Kitchen, Gareth B. et al. The clock gene Bmal1 inhibits macrophage motility, phagocytosis, and impairs defense against pneumonia. Proceedings of the National Academy of Sciences of the United States of America. 2020
Angelini, Aude et al. Cardioprotective effects of α-cardiac actin on oxidative stress in a dilated cardiomyopathy mouse model. FASEB Journal. 2020
Shi, Zengdun et al. Myocardin and myocardin-related transcription factor-A synergistically mediate actin cytoskeletal-dependent inhibition of liver fibrogenesis. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2020
Huang, Rui et al. NCAM regulates temporal specification of neural progenitor cells via profilin2 during corticogenesis. Journal of Cell Biology. 2020
Hogrebe, Nathaniel J. et al. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells. Nature Biotechnology. 2020
Luo, Xi et al. Vascular NRP2 triggers PNET angiogenesis by activating the SSH1-cofilin axis. Cell and Bioscience. 2020
Chen, Yuewen et al. Coronin 2B regulates dendrite outgrowth by modulating actin dynamics. FEBS Letters. 2020
Li, Huitao et al. NC1-Peptide from collagen α3 (IV) chains in the basement membrane of testes regulates spermatogenesis via p-FAK-Y407. Endocrinology (United States). 2020
Bojcevski, Jovana et al. Influence of retinal NMDA receptor activity during autoimmune optic neuritis. Journal of Neurochemistry. 2020
Sankaran, Jeyantt S. et al. Knockdown of formin mDia2 alters lamin B1 levels and increases osteogenesis in stem cells. Stem Cells. 2020
Lu, Yi Ju et al. Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity. Nature Communications. 2020
Yang, Nuo et al. Effects of dexamethasone on remodeling of the hippocampal synaptic filamentous actin cytoskeleton in a model of pilocarpine-induced status epilepticus. International Journal of Medical Sciences. 2020
Jansson, Thomas et al. Down-regulation of placental Cdc42 and Rac1 links mTORC2 inhibition to decreased trophoblast amino acid transport in human intrauterine growth restriction. Clinical Science. 2020
Burger, Joyce et al. Fibulin-4 deficiency differentially affects cytoskeleton structure and dynamics as well as TGFβ signaling. Cellular Signalling. 2019
Wenceslau, Camilla F. et al. Formyl peptide receptor-1 activation exerts a critical role for the dynamic plasticity of arteries via actin polymerization. Pharmacological Research. 2019
Childers, Rachel C. et al. Role of the cytoskeleton in the development of a hypofibrotic cardiac fibroblast phenotype in volume overload heart failure. American Journal of Physiology - Heart and Circulatory Physiology. 2019
Torrini, Consuelo et al. Common Regulatory Pathways Mediate Activity of MicroRNAs Inducing Cardiomyocyte Proliferation. Cell Reports. 2019
Wen, Qing et al. Myosin VIIa supports spermatid/organelle transport and cell adhesion during spermatogenesis in the rat testis. Endocrinology. 2019
Wang, Yan et al. CD44 inhibits α-SMA gene expression via a novel G-actin/MRTF-mediated pathway that intersects with TGFβR/ p38MAPK signaling in murine skin fibroblasts. Journal of Biological Chemistry. 2019
Rockey, Don C. et al. Smooth Muscle α-Actin Deficiency Leads to Decreased Liver Fibrosis via Impaired Cytoskeletal Signaling in Hepatic Stellate Cells. American Journal of Pathology. 2019
Zhao, Fei et al. Exendin-4 promotes actin cytoskeleton rearrangement and protects cells from Nogo-A-Δ20 mediated spreading inhibition and growth cone collapse by down-regulating RhoA expression and activation via the PI3K pathway. Biomedicine and Pharmacotherapy. 2019
Yu, Minhao et al. Increased proton-sensing receptor GPR4 signalling promotes colorectal cancer progression by activating the hippo pathway. EBioMedicine. 2019
Miller, Karl N. et al. PGC-1a integrates a metabolism and growth network linked to caloric restriction. Aging Cell. 2019
Yuki, Kyoko E. et al. CYRI/FAM49B negatively regulates RAC1-driven cytoskeletal remodelling and protects against bacterial infection. Nature Microbiology. 2019
Hu, Fang et al. Nogo-A promotes inflammatory heat hyperalgesia by maintaining TRPV-1 function in the rat dorsal root ganglion neuron. FASEB Journal. 2019
Chen, Wei et al. The phenotype of peritoneal mouse macrophages depends on the mitochondria and ATP/ADP homeostasis. Cellular Immunology. 2018
Chiu, Amy Pei Ling et al. Dual effects of hyperglycemia on endothelial cells and cardiomyocytes to enhance coronary LPL activity. American Journal of Physiology - Heart and Circulatory Physiology. 2018
Zhang, Fanrong et al. GAP43, a novel metastasis promoter in non-small cell lung cancer 11 Medical and Health Sciences 1112 Oncology and Carcinogenesis. Journal of Translational Medicine. 2018
Cahill, Michael E. et al. Withdrawal from repeated morphine administration augments expression of the RhoA network in the nucleus accumbens to control synaptic structure. Journal of Neurochemistry. 2018
Wen, Qing et al. Actin nucleator Spire 1 is a regulator of ectoplasmic specialization in the testis. Cell Death and Disease. 2018
Bardai, Farah H. et al. A conserved cytoskeletal signaling cascade mediates neurotoxicity of FTDP-17 tau mutations in vivo. Journal of Neuroscience. 2018
Kommaddi, Reddy Peera et al. Aβ mediates F-actin disassembly in dendritic spines leading to cognitive deficits in alzheimer’s disease. Journal of Neuroscience. 2018
Laguesse, Sophie et al. Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward. Neuron. 2017
Mauriac, Stephanie A. et al. Defective Gpsm2/Gαi3 signalling disrupts stereocilia development and growth cone actin dynamics in Chudley-McCullough syndrome. Nature Communications. 2017
González-Jamett, Arlek M. et al. Dynamin-2 mutations linked to Centronuclear Myopathy impair actin-dependent trafficking in muscle cells. Scientific Reports. 2017
Halim, Danny et al. Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice. Proceedings of the National Academy of Sciences of the United States of America. 2017
Tavares, Sandra et al. Actin stress fiber organization promotes cell stiffening and proliferation of pre-invasive breast cancer cells. Nature Communications. 2017
Allison, Patrick et al. BMP2 rescues deficient cell migration in Tgfbr3−/− epicardial cells and requires Src kinase. Cell Adhesion and Migration. 2016
Pérez-Núñez, Ramón et al. Overexpressed Down Syndrome Cell Adhesion Molecule (DSCAM) Deregulates P21-Activated Kinase (PAK) Activity in an In Vitro Neuronal Model of Down Syndrome: Consequences on Cell Process Formation and Extension. Neurotoxicity Research. 2016
Zhang, Shu Xin et al. Actin Aggregations Mark the Sites of Neurite Initiation. Neuroscience Bulletin. 2016
Peykov, S. et al. Identification and functional characterization of rare SHANK2 variants in schizophrenia. Molecular Psychiatry. 2015
Caution, Kyle et al. Caspase-11 and caspase-1 differentially modulate actin polymerization via RhoA and Slingshot proteins to promote bacterial clearance. Scientific Reports. 2015
Freeman, Spencer A. et al. Toll-like receptor ligands sensitize B-cell receptor signalling by reducing actin-dependent spatial confinement of the receptor. Nature Communications. 2015
Han, Yu et al. Noise-induced cochlear F-actin depolymerization is mediated via ROCK2/p-ERM signaling. Journal of Neurochemistry. 2015
Allison, Patrick et al. Type III TGFβ receptor and Src direct hyaluronan-mediated invasive cell motility. Cellular Signalling. 2015
Kwok, Willson et al. Rho-kinase activation contributes to lps-induced impairment of endothelial nitric oxide synthase activation by endothelin-1 in cultured hepatic sinusoidal endothelial cells. Shock. 2014
Lockett, Stephen et al. Quantitative analysis of F-actin redistribution in astrocytoma cells treated with candidate pharmaceuticals. Cytometry Part A. 2014
Huang, Xun et al. JG6, a novel marine-derived oligosaccharide, suppresses breast cancer metastasis via binding to cofilin. Oncotarget. 2014
Wang, J. et al. Erratum: The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus (Acta Pharmacol Sin (2013) 34 (811-8) Doi: 10.1038/aps.2013.3 ). Acta Pharmacologica Sinica. 2014
Ramachandran, Aruna et al. JunB Mediates Basal- and TGFβ1-Induced Smooth Muscle Cell Contractility. PLOS ONE. 2013
Ni, Jixiang et al. The role of RhoA and cytoskeleton in myofibroblast transformation in hyperoxic lung fibrosis. Free Radical Biology and Medicine. 2013
Shuang, Feng et al. Destrin deletion enhances the bone loss in hindlimb suspended mice. European journal of applied physiology. 2013
Asahara, S. et al. Ras-related C3 botulinum toxin substrate 1 (RAC1) regulates glucose-stimulated insulin secretion via modulation of F-actin. Diabetologia. 2013
Stehn, Justine R. et al. A novel class of anticancer compounds targets the actin cytoskeleton in tumor cells. Cancer Research. 2013
Agrawal, Pankaj B. et al. Normal myofibrillar development followed by progressive sarcomeric disruption with actin accumulations in a mouse Cfl2 knockout demonstrates requirement of cofilin-2 for muscle maintenance. Human Molecular Genetics. 2012
Fan, Jianguo et al. A role for γs-crystallin in the organization of actin and fiber cell maturation in the mouse lens. FEBS Journal. 2012
Varol, Başak et al. The cytotoxic effect of diphtheria toxin on the actin cytoskeleton. Cellular and Molecular Biology Letters. 2012
Durand, C. M. et al. SHANK3 mutations identified in autism lead to modification of dendritic spine morphology via an actin-dependent mechanism. Molecular Psychiatry. 2012
Malenda, Agata et al. Statins Impair Glucose Uptake in Tumor Cells. Neoplasia. 2012
Liu, Shirong et al. TLR2 is a primary receptor for Alzheimer's amyloid β peptide to trigger neuroinflammatory activation. Journal of immunology (Baltimore, Md. : 1950). 2012
Shen, Dongxiao et al. Aortic aneurysm generation in mice with targeted deletion of integrin-linked kinase in vascular smooth muscle cells. Circulation Research. 2011
Rapier, Rebecca et al. The extracellular matrix microtopography drives critical changes in cellular motility and Rho A activity in colon cancer cells. Cancer Cell International. 2010
Ambrosino, Concetta et al. Identification of a hormone-regulated dynamic nuclear actin network associated with estrogen receptor α in human breast cancer cell nuclei. Molecular and Cellular Proteomics. 2010
Du, Hui et al. Phenylephrine induces elevated RhoA activation and smooth muscle α-actin expression in Pkd2+/-vascular smooth muscle cells. Hypertension Research. 2010
Romero, Ana M. et al. Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary culture. Toxicological Sciences. 2010
Tejani, Ankit D. et al. Force augmentation and stimulated actin polymerization in swine carotid artery. American Journal of Physiology - Cell Physiology. 2010
McArthur, Simon et al. Annexin A1 regulates hormone exocytosis through a mechanism involving actin reorganization. FASEB Journal. 2009
Fu, Xiao Dong et al. Extra-nuclear signaling of progesterone receptor to breast cancer cell movement and invasion through the actin cytoskeleton. PLoS ONE. 2008
Bergstrom, R. A. et al. Agrin induced morphological and structural changes in growth cones of cultured hippocampal neurons. Neuroscience. 2007
McCarthy, Alicia M. et al. Loss of cortical actin filaments in insulin-resistant skeletal muscle cells impairs GLUT4 vesicle trafficking and glucose transport. American Journal of Physiology - Cell Physiology. 2006
Meeks, Melissa K. et al. Heat shock protein 20-mediated force suppression in forskolin-relaxed swine carotid artery. American journal of physiology. Cell physiology. 2005
Sebastian, Becky M. et al. Decreased insulin-dependent glucose transport by chronic ethanol feeding is associated with dysregulation of the Cbl/TC10 pathway in rat adipocytes. American Journal of Physiology - Endocrinology and Metabolism. 2005
Zhang, Wenwu et al. Activation of the Arp2/3 complex by N-WASp is required for actin polymerization and contraction in smooth muscle. American journal of physiology. Cell physiology. 2005
Searles, Charles D. et al. Actin cytoskeleton organization and posttranscriptional regulation of endothelial nitric oxide synthase during cell growth. Circulation research. 2004
Tang, Dale D. et al. The small GTPase Cdc42 regulates actin polymerization and tension development during contractile stimulation of smooth muscle. Journal of Biological Chemistry. 2004
Chen, Guoli et al. Protective effect of phosphatidylinositol 4,5-bisphosphate against cortical filamentous actin loss and insulin resistance induced by sustained exposure of 3T3-L1 adipocytes to insulin. Journal of Biological Chemistry. 2004
Tang, Dale D. et al. Role of Crk-Associated Substrate in the Regulation of Vascular Smooth Muscle Contraction. Hypertension. 2003
Tu, Yizeng et al. Migfilin and Mig-2 link focal adhesions to filamin and the actin cytoskeleton and function in cell shape modulation. Cell. 2003
Cenni, V. et al. Targeting of the Akt/PKB kinase to the actin skeleton. Cellular and Molecular Life Sciences. 2003
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Overview