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Overview
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Product Uses Include:
Arf1 signaling pathway studies
Arf1 activation assays with primary cells
Studies of Arf1 activators and inactivators
Arf1 activation assays with limited material
High throughput screens for Arf1 activation
Introduction
With the new Arf1 G-LISA® kit you can now measure Arf1 activation from cell and tissue samples in less than 3 h. G-LISA® requires only 1-5% of the material needed for a conventional pull-down assay. You will also be able to handle large sample numbers and generate quantitative results. For a more detailed introduction on G-LISA® assays and a listing of other available G-LISA® kits, see our main G-LISA page.
The Arf1 G-LISA® kit contains an Arf-GTP-binding protein linked to the wells of a 96 well plate. Active, GTP-bound Arf1 in cell/tissue lysates will bind to the wells while inactive GDP-bound Arf1 is removed during washing steps. The bound active Arf1 is detected with a Arf1 specific antibody. The degree of Arf1 activation is determined by comparing readings from activated cell lysates versus non-activated cell lysates.Please contact us at for specific academic pricing.
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Overview