ArcticZymes R2D Ligase™

ArcticZymes R2D Ligase™ is a unique ATP dependent double-strand DNA ligase able to join RNA to DNA.
ArcticZymes R2D Ligase™ is the first ligase on the market that is able to ligate DNA to 5’-phosphorylated ends of RNA in the presence of a DNA template positioning the joinable ends. With its unique substrate specificity, ArcticZymes R2D Ligase allows the development of new technologies in molecular biology research, diagnostics, and manufacturing.

Unit Definition: One milliunit is defined as the amount of enzyme needed to ligate 1 pmol (of 18 pmol) of a nicked DNA substrate in 20 minutes at 25 °C in a 20 µl reaction volume in a buffer consisting of 62.5 mM Tris-HCl, pH 7.5 (25°C), 5 mM MgCl2, 1 mM ATP, 10 mM DTT, 0.025 mg/ml BSA and 25 mM KCl.

R2D DNA Ligase is manufactured at our ISO 13485 certified facility in Norway.

dsDNA endonuclease activity
2.5 U R2D Ligase was incubated with a supercoiled plasmid (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any transformation of closed circular DNA to nicked DNA.

ssDNA endonuclease activity
2.5 U R2D Ligase was incubated with M13 ssDNA (0.5 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of ssDNA degradation.

Exonuclease activity
2.5 U R2D Ligase was incubated with either 3H-dATP labelled ds or ssDNA (0.5 µg, 500 bp) for 4 hours at 37°C. Acid soluble radioactivity from labelled DNA was not significantly over blank test for either substrate.

RNase activity
2.5 U R2D Ligase was incubated with a 2 kb RNA transcript (1 µg) for 4 hours at 37°C. Agarose gel electrophoresis did not reveal any visible signs of RNA degradation.

E. coli gDNA contamination
2.5 U R2D Ligase was analysed in a probe-based qPCR assay detecting the 23S ribosomal subunit in E. coli. No E. coli gDNA could be detected (LOD: < 3 E. coli genomic copies).

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