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Overview
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This recombinant human adenovirus type 5 expresses enhanced green fluorescent protein (GFP) under the CAG (also known as CBA) promoter. Green fluorescent protein (GFP) is a protein originally isolated from the jellyfish that fluoresces green when exposed to blue light. Enhanced GFP (eGFP) is a GFP mutant with improved fluorescence and stability. Recombinant eGFP adenovirus serves as a control for other recombinant adenoviruses. Since GFP is easily visualized under fluorescence microscopy, this adenovirus can also be used to determine the transduction efficiency and to optimize viral infection condition in a specific cell type.
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