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Overview
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Product Uses Include:
Microinjection into muscle cell followed by electron microscopy of streptavidin conjugated gold particles to determine the cellular localization of the modified actin.
in vitro motility experiments using biotinylated seeds of F-actin bound to fluorescent beads for laser tweezer manipulation.
Produce affinity columns using biotinylated F-actin or G-actin to extract and purify novel proteins.
Purify proteins or develop bioassays using biotin F-actin or G-actin with streptavidin-magnetic beads.
Material
Rabbit skeletal muscle actin (Cat. # AKL99) has been modified to contain covalently linked biotin at random surface lysine residues. An activated ester of biotin is used to label the protein. The labeling stoichiometry has been determined to be approximately 1 biotin per actin monomer. Biotinylated actin has an approximate molecular weight of 43 kDa. AB07 is supplied as a lyophilized powder. The lyophilized protein when stored desiccated to < 10% humidity at 4°C is stable for 6 months. The protein should be reconstituted to 10 mg/ml with distilled water. The protein will then be in the following buffer: 5 mM Tris-HCl pH 8.0, 0.2 mM CaCl2, 0.2 mM ATP, 5% sucrose, and 1% dextran.Please contact us at for specific academic pricing.
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Overview