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Overview
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AAV1-CAG-Mito-GFP is a pre-packaged rAAV in serotype 1 (with capsid from AAV serotype 1 and 2xITR from AAV serotype 2) which expresses the Mito-EGFP fusion protein under the CAG promoter (also known as the CBA promoter) The CAG promoter is a combination of the cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter for high levels of gene expression in mammalian cells. The mitochondrial targeting sequence from subunit VIII of human cytochrome C oxidase (Mito) is fused with N-terminal of the EGFP. The Mito sequence targets the Mito-EGFP fusion protein to the host cells mitochondria.
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- Properties
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Overview