Flow cytometry is a single-particle analysis technique that measures particles aligned in a central stream by sheath flow within a microchannel. Single-particle analysis is achieved through the measurement of fluorescence signals and scattered light signals in two directions. Conventional flow cytometry is an effective technique for measuring the size, concentration, and phenotype of micron-sized particles with high accuracy and reproducibility. Nanoscale flow cytometry is a new technology that solves the limitations of nanoscale analysis of conventional flow cytometry through technical optimization. This new technology can be applied to the study of extracellular vesicles, lipid nanoparticles, viruses, bacteria, and nanoscale organelles and biological particles. It allows the use of lower solution volumes and bring many benefits to the quantitative and qualitative analysis of nanoparticles.

Nanoscale Flow Cytometer for the Analysis of 5-3000 nm Nanoparticles

Amerigo Scientific offers the Exoplorer™ nano-flow cytometer designed primarily for the characterization of biological nanoparticles, including extracellular vesicles, viruses, lipid nanoparticles, bacteria, subcellular subunits, and other nanoparticles.

The Exoplorer™ nanoflow cytometer offers high sensitivity, resolution, and throughput for comprehensive analysis of nanoparticles in the 5-3000 nm range. Its functionality extends to size distribution, particle counting, and biochemical characterization assessment, making it a powerful tool for life science and biomedical research.

Features and Advantages

Four scatter channels

• Four scatter channels: VSSC-1, VSSC-2, FSC, and BSSC
• Detectable particle range: 5nm-3000nm
• Supporting three signal parameters (area, height, and width), effectively distinguishing system background and noise, ensuring accurate optical signal capture

Dual lasers and eight fluorescence channels

• Featuring violet and blue dual lasers and supporting up to eight fluorescence channels
• Accurate detecting each signal peak from 8-peak beads

Sizing Reference

• A standard algorithm is used for curve fitting to calculate particle size. Results are given in nm and the refractive index of the biological sample is used to determine the size.

Bead-free Absolute Counting

• Featuring exceptional consistency, stability, and reliability in absolute counting functionality

Multiple Fluorescent Markers Supported

• Empowering multicolor schemes and elevating multicolor experiments

Strong Robust System Stability

• Mean fluorescence intensity variation within ±5% for 30 consecutive days
• Operational temperature range: 15-28 °C; fluctuation±°C

User-Friendly Design

• Test tube types for sample station: 5 ml flow cytometry tube, 1.5 ml and 2.0 ml EP tube, and liquid container carriage of any height
• Maximum 50μL sample aspirated at a time consecutively
• Carryover rate <0.1%, exceeding industry standards
• Compatible with BSL-2 biosafety cabinet in size and weight
• Analysis speed: up to 34,000 events per second
• Electronic signature in accord with the 21CFR
• Exportable FCS files, compatible with third-party software

Applications