Biological safety cabinets (BSCs), also known as biosafety cabinets, are enclosed, ventilated working spaces that can be used in laboratories as primary containment devices. These devices are designed to protect the operator, the laboratory environment and/or the work materials from exposure to infectious aerosols and splashes that may be generated when manipulating materials containing biological agents. According to different research needs, BSCs are divided into three categories, designated as Class I, Class II, and Class III. Class II and Class III cabinets provide operator, product, and environmental protection, while the Class I cabinets provide operator and environmental protection but no product protection. All BSCs use high efficiency particulate air (HEPA) filters to capture and filter particulate matter in intake and exhaust air. The HEPA filter is composed of many randomly oriented fibers that form a fibrous matrix. When air passes through the HEPA filter, particles that travel with the air can be captured by the fibers, thus effectively filtering the air. Most HEPA filters have a filtering efficiency of more than 99.97% for particles with a diameter of 0.3µm, which is the most penetrating particle size. Particles with a lower or higher diameter are removed with higher efficiency.
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