Cell Processing Systems

As more cell-based biological products are widely applied, the process of scale-up production of high-quality cells is very essential due to the need for a large number of high-quality cells. Different types of suspension and adherent cells have very different phenotypes and characteristics. To scale up production and meet production standards, cells need to be cultured in a controlled and monitored environment. Various bioreactors such as stirred tank bioreactors, bubble column bioreactors and airlift bioreactors are designed for the culture of a wide range of different cell types with different culture requirements. Downstream processing in cell manufacturing consists of the post-culture treatment of cells, which generally includes cell harvesting, cell washing and concentration, medium replacement, and cell filling.

Cell harvesting refers to the process of extracting cells from the final culture containers, which depends on the type of container, culture technique, surface chemistry, and cell type. Cells must be collected with minimal damage, and the harvesting method varies depending on the culture method. Cell detachment is essential in harvesting adherent cells. Enzymatic detachment is the most commonly used mothed, which is generally performed using recombinant animal-origin-free proteases. In addition to enzymes, mechanical forces are often added to physically remove cells from the surface. Although the addition of physical force during harvesting is essential for cell release, in many cases it transmits shear forces to cells, which can damage the cells. Therefore, it is critical to optimize the harvesting process by reducing shear stress, cell damage, and cell clump formation, while maintaining the highest cell yield. In addition to enzymatic digestion methods, other methods include covering the carrier surface with a material that changes its chemistry in response to temperature changes to release cells, and carriers that are biodegradable or dissolve with temperature changes. Since most cell products consist of single-cell suspensions, the filtration step is critical to eliminate any cell clumps or particulates after cell harvesting. The solution after cell harvesting usually contains culture medium, serum, enzymes, cell debris, etc. Therefore, the next step in cell processing is buffer exchange, cell concentration, and washing of ancillary materials. The formulation step is the process of adjusting the cell concentration and volume, which may also need to be performed depending on the method of storage and delivery of the cells. Finally, filling the final cell product into the final container is a critical step of downstream processing, where cells should be suspended in the final storage solution. During cell processing, it is necessary to minimize the temperature variation and keep cells uniformly dispersed and free from contamination.

Amerigo Scientific provides high-performance, scalable, and reliable instruments for cell harvesting, cell filling, and other cell processing. Our systems are designed to produce high-quality cells with high efficiency and low cost.

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