The DIANT® LARU – Benchtop LNP Processing

Solvent Injection
The LARU features DIANT® Jet technology, a patented single-jet, low flow rate, low dead volume (LDV) solvent injection system for high throughput.

Process Analytical Technology (PAT) via NanoFlowSizer™
As the exclusive distributor of the NanoFlowSizer™ in the United States, DIANT's systems work with InProcess-LSP's unique particle size analyzer technology in order to non-invasively characterize nanoparticles inline:
Integrated, inline particle size control and monitoring with InProcess-LSP's NanoFlowSizer™, SR-DLS
Automatic particle size control can enable rapid runs of 20 seconds for determining optimal particle size for your experiments
Real-time particle monitoring, such as inline lipid analysis

Multiple Program Options
Store and create runtime recipes for producing particles under different flow conditions and set durations.

DIANT® LARU Add-Ons and Continuous Processing Systems
Optional add-ons that are available for the DIANT® LARU include:
· Customized PAT integration
· Particle size analyzer
· pH monitor/probes
· Conductivity
· Temperature control – Control the temperature of your sample at each stage with the water circulator, sold separately

DIANT® LARU + 2TFF
· The 2TFF uses a proprietary multi-stage tangential flow filtration system to reduce biofouling and allow for long duration runs. Concentrates particles using a closed system, operating in single-pass mode.

DIANT® LARU + IEM
The DIANT® IEM is a continuous processing system for the
controlled modification of pre-formed vesicular nanoparticles:
· Intravesicular aqueous modifications – small molecules like liposomes can be actively loaded to promote drug encapsulation
· Extravesicular surface modifications – capabilities include adding polymeric coating and active drug moieties

The IEM system can:
Perform one or several simultaneous nanoparticle modifications as a continuous process
PEGylate particles, including LNPs, polymeric micelles, and others
Decorate particles
Manage growth kinetics
· Salt loading for liposomes
· Surface deposition for LNPs
Perform PAT – inline UV-Vis with model prediction (optional & customizable)
Sense pressure, conductivity, and temperature
Perform inline heat exchange and mixing
Perform inline bioburden reduction filtration at collection
Perform inline single-pass concentration

· Scales up from R&D to commercial cGMP manufacturing
· Performs continuous or batch operations
· Produces nanoparticles of various sizes: ~25 d.nm to >500 d.nm
· Collects volumes as low as 20 mL (short runs)
· Has standard connections for bottles, tubes, or 2D bags for ease of use

The LARU is a pilot-scale system that can continuously produce lipid nanoparticles (LNPs), liposomes, nucleic acid/lipid complexes, polymeric micelles, suspensions, and emulsions.