Protein G Bestarose 4FF - Antibody Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502255-01	EzFast Protein G 4FF Prepacked column	1×1mL	Inquiry
CC02502255-02	EzFast Protein G 4FF Prepacked column	1×5mL	Inquiry
CC02502255-03	EzFast Protein G 4FF Prepacked column	5×1mL	Inquiry
CC02502255-04	EzFast Protein G 4FF Prepacked column	5×5mL	Inquiry
CC02502255-05	EzScreen Protein G 4FF Prepacked column	1×4.6mL	Inquiry
CC02502255-06	EzFast Protein G 4FF Prepacked column	5×4.6mL	Inquiry
CC02502255-07	EzLoad 16/10 Protein G 4FF Prepacked column	1 pcs	Inquiry
CC02502255-08	EzLoad 26/10 Protein G 4FF Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Protein G Bestarose 4FF is an affinity resin made by fixing Protein G to Bestarose 4FF base matrix by cyanogen bromide activation. Protein G has a broader binding spectrum than Protein A. At the same time, Protein G has strong binding to the Fc fragment of the antibody, while having weak interaction with the Fab fragment of the antibody, so Protein G Bestarose 4FF is often used for separation Purification of antibodies or antibody fragments from cell culture media and purification of antibodies from sera of various species.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked agarose, 4%
Particle size +	45-165μm
Functional group	Protein G (E.coli)
Ligand concentration	~2mg Protein G /mL resin
Cross-linking method	Cyanogen bromide activation
Dynamic binding capacity++	~20mg human IgG /mL packed resin
Chemical stability	40°C,1 week: 1M HAc (pH2.0),20mM sodium phosphate, 1%SDS, 6M GuHCl, 70% ethanol At room temperature 2h: 0.1 M HCl (pH1.0), 8M Urea (pH10.5)
Physical stability	Volume changes due to changes in pH or ionic strength are negligible
Max. pressure	0.3MPa
Pressure flow velocity	150~250cm/h (0.1MPa BXK50, H=25cm)
pH stability	3~9(working), 2~10 (CIP)
Storage+++	2~8°C,20% ethanol or 2% benzyl alcohol

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total
++ Linear flow velocity 200cm / h, bed height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH 7.4
+++2% benzyl alcohol is only used for international transport or special requirements from customer.