IMAC Bestarose HP - Tag Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502256-01	EzFast IMAC HP Prepacked column	1×1mL	Inquiry
CC02502256-02	EzFast IMAC HP Prepacked column	5×1mL	Inquiry
CC02502256-03	EzFast IMAC HP Prepacked column	1×5mL	Inquiry
CC02502256-04	EzFast IMAC HP Prepacked column	5×5mL	Inquiry
CC02502256-05	EzScreen IMAC HP Prepacked column	1×4.6mL	Inquiry
CC02502256-06	EzScreen IMAC HP Prepacked column	5×4.6mL	Inquiry
CC02502256-07	EzLoad 16/10 IMAC HP Prepacked column	1 pcs	Inquiry
CC02502256-08	EzLoad 26/10 IMAC HP Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

IMAC Bestarose HP (High Performance) is a high-resolution metal chelating affinity chromatography resin, combined with a variety of transition metal ions such as Cu2+, Zn2+, Ni2+, Co2+, Fe3+, etc, which can be used to separate and purify proteins and peptides. The principle is to use the interaction between the side chains of histidine, cysteine and tryptophan of the protein and metal ions, so as to achieve the purpose of separation and purification.
IMAC Bestarose HP is formed by cross-linking agarose and nitrilotriacetic acid (NTA). It can chelate the four-valent position of metal ions, making the chelated metal ions more stable, as well as tolerate higher reducing agents. It also enjoys good chemical stability, good specificity and fast flow velocity.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked agarose, 6%
Particle size +	24~44μm(the average particle size is 34μm)
Chelating ability	~15μmol Ni2+/mL resin
Dynamic binding capacity	~ 40mg His tag protein/ mL packed resin(chelating nickel ion)
Chemical stability ++	1 week at 40°C: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl, 40°C 12h: 1M NaOH, 70% acetic acid.
Max. pressure	0.3MPa
pH stability+++	3~12(working) ,2~14(CIP)
Storage++++	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	＜150cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++ Stability when removing metal ions.
+++ CIP refers to pH stability when metal ions are removed.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.