GST Bestarose 4B - Tag Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502258-01	EzFast GST 4B Prepacked column	1×1mL	Inquiry
CC02502258-02	EzFast GST 4B Prepacked column	1×5mL	Inquiry
CC02502258-03	EzFast GST 4B Prepacked column	5×1mL	Inquiry
CC02502258-04	EzFast GST 4B Prepacked column	5×5mL	Inquiry
CC02502258-05	EzScreen GST 4B Prepacked column	1×4.6mL	Inquiry
CC02502258-06	EzScreen GST 4B Prepacked column	5×4.6mL	Inquiry
CC02502258-07	EzLoad 16/10 GST 4B Prepacked column	1 pcs	Inquiry
CC02502258-08	EzLoad 26/10 GST 4B Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

GST Bestarose 4B is an affinity resin made by coupling glutathione to agarose, which is used to specifically purify glutathione S-transferase (GST) and GST fusion protein. GST tag is a tag commonly used in genetic engineering to express fusion proteins, which facilitates soluble expression and activity maintenance of proteins. For different sources of glutathione S-transferase and its fusion protein, this resin can be used to obtain high-purity target protein in one step of purification. The chromatographic resin has mild operating conditions and helps the maintenance of protein activity.

Specifications

Appearance	White slurry
Matrix	4% agarose
Particle size+	45~165μm
Functional group	Glutathione with 10 atomic arms
Dynamic binding capacity	＞5mg GST/mL packed resin
Max. pressure	0.16 bar
Operation flow velocity	~75cm/h (BXP16/30 column, H=5cm,25°C)
Chemical stability	Stable in common aqueous buffers: 1M HAc++, 0.1M NaOH, 70% ethanol, 6M GuHCl (room temperature for 2 hours)
pH stability	4~13
Operating temperature	Operating temperature: 2~30°C, do not freeze
Storage+++	2~30°C,20% ethanol or 2% benzyl alcohol

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.