Ni Bestarose HP - Tag Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502267-01	EzFast Ni HP Prepacked column	1×1mL	Inquiry
CC02502267-02	EzFast Ni HP Prepacked column	1×5mL	Inquiry
CC02502267-03	EzFast Ni HP Prepacked column	5×1mL	Inquiry
CC02502267-04	EzFast Ni HP Prepacked column	5×5mL	Inquiry
CC02502267-05	EzScreen Ni HP Prepacked column	1×4.6mL	Inquiry
CC02502267-06	EzScreen Ni HP Prepacked column	5×4.6mL	Inquiry
CC02502267-07	EzLoad 16/10 Ni HP Prepacked column	1 pcs	Inquiry
CC02502267-08	EzLoad 26/10 Ni HP Prepacked column	1 pcs	Inquiry
CC02502267-09	EzLoad 26/20 Ni HP(Double adaptor） Prepacked column	1 pcs	Inquiry

Add to Cart

Product Description

Product Description

Ni Bestarose HP (High Performance) metal chelation chromatography resin is an affinity chromatography resin made by pre-chelating metal ion Ni2+ on a high-resolution agarose with NTA as ligand. It has the advantages of high resolution, large adsorption capacity, good selectivity, easy regeneration, and low cost. It is widely used in the separation and purification of downstream proteins and peptides in biopharmaceutical and bioengineering, especially the efficient purification of histidine-tagged proteins.

Specifications

Appearance	Turquoise slurry
Matrix	Cross-linked agarose, 6%
Particle size +	24~44μm(the average particle size is 34μm)
Ligand concentration	~15μmol Ligand/mL resin
Dynamic binding capacity	~ 40mg His tag protein/mL packed resin
Chemical stability ++	1 week at 40°C: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl 40°C 12h: 1M NaOH, 70% acetic acid
Max. pressure	0.3MPa
pH stability+++	3~12(working) 2~14(CIP)
Storage++++	2~30°C,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	＜150cm/h

+Particle size is the cumulative resin particle size of packing volume distribution.
++ Stability when removing metal ions.
+++ CIP refers to pH stability when metal ions are removed.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.