Heparin Bestarose HP - Affinity Chromatography Columns

Cat. No. Specification Unit Price Quantity
CC02502269-01 EzFast Heparin HP Prepacked column 1×1mL Inquiry
CC02502269-02 EzFast Heparin HP Prepacked column 1×5mL Inquiry
CC02502269-03 EzFast Heparin HP Prepacked column 5×1mL Inquiry
CC02502269-04 EzFast Heparin HP Prepacked column 5×5mL Inquiry
CC02502269-05 EzScreen Heparin HP Prepacked column 1×4.6mL Inquiry
CC02502269-06 EzScreen Heparin HP Prepacked column 5×4.6mL Inquiry
CC02502269-07 EzLoad 16/10 Heparin HP Prepacked column 1 pcs Inquiry
CC02502269-08 EzLoad 26/10 Heparin HP Prepacked column 1 pcs Inquiry

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Product Description
Product Description
Heparin Bestarose HP (High Performance) is a family-specific affinity chromatography resin made by covalently coupling heparin to fine particles with high cross-linking agarose. Widely used in the separation and purification of various biological molecules, especially various enzymes, including antithrombin III, thrombin-like, human coagulation factor IX, XI, VIII, lipoprotein lipase, collagenase, DNA polymerase. It can also bind human interleukin, human prostate growth factor, recombinant human vascular endothelial growth factor, cartilage growth factor, basic fibroblast growth factor, recombinant human acid fibroblast growth factor, recombinant hepatocyte growth factor, and recombinant murine heparin cofactor II, recombinant human platelet fourth factor, recombinant human endostatin, recombinant human keratinocyte growth factor and other biological macromolecules. The resin has the characteristics such as high resolution, physical and chemical stability, firmly attached ligands, long service life, and a wide range of applications.
Specifications

Appearance

White slurry

Matrix

Highly cross-linked 6% agarose

Particle size+

24~44μm (the average particle size is 34μm)

Functional group

Heparin

Ligand concentration

~10mg Heparin Ligand/mL resin

Dynamic binding capacity

2-5 mg bovine AT III/ mL packed resin

Chemical stability

Stable in common aqueous buffers: 8M urea, 6M GuHCl, 70% ethanol, 50mM sodium acetate(pH4), 10% glycerin, 0.1M NaOH (20°C, a week)

pH stability

5~10

Storage++

2~30°C, 20% ethanol + 50mM NaAc or 2% benzyl alcohol +50mM NaAc

Recommended flow velocity

<150cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++2% benzyl alcohol is only used for international transport or special requirements from customer.
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