GST Bestarose 4FF - Tag Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502259-01	EzFast GST 4FF Prepacked column	1×1mL	Inquiry
CC02502259-02	EzFast GST 4FF Prepacked column	1×5mL	Inquiry
CC02502259-03	EzFast GST 4FF Prepacked column	5×1mL	Inquiry
CC02502259-04	EzFast GST 4FF Prepacked column	5×5mL	Inquiry
CC02502259-05	EzScreen GST 4FF Prepacked column	1×4.6mL	Inquiry
CC02502259-06	EzScreen GST 4FF Prepacked column	5×4.6mL	Inquiry
CC02502259-07	EzLoad 16/10 GST 4FF Prepacked column	1 pcs	Inquiry
CC02502259-08	EzLoad 26/10 GST 4FF Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

GST Bestarose 4FF is made by coupling glutathione to a highly cross-linked agarose, which is used to specifically purify glutathione S-Transferase (GST) as well as the purification of GST fusion protein. GST tag is a tag commonly used in modern genetic engineering to express fusion proteins, allowing soluble expression and activity maintenance of proteins. For different sources of glutathione S-transferase and its fusion proteins, High-purity target protein can be obtained in one-step purification when using this resin. The c resin has high pressure resistance, fast flow velocity, and mild operating conditions, which can help with maintenance of protein activity.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked agarose, 4%
Particle size+	45-165μm
Functional group	Glutathione with 10 atomic arms
Ligand concentration	120~320μmol Glutathione /mL resin
Dynamic binding capacity	~10mg GST/mL packed resin
Max. pressure	0.3MPa
Chemical Stability	Stable in common aqueous buffers: 1M HAc++, 70%ethanol, 6M GuHCl (1 hour at room temperature)
pH stability	3~12
Storage+++	2~30°C, 20%ethanol or 2% benzyl alcohol
Pressure flow velocity	~450cm/h, BXK16/10,H=5cm,25°C
Recommended flow velocity	Sample loading flow velocity:＜100cm/h Equilibration, cleaning, elution flow velocity:100~300cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.