Ni Bestarose FF - Tag Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502261-01	EzFast Ni FF Prepacked column	1×1mL	Inquiry
CC02502261-02	EzFast Ni FF Prepacked column	1×5mL	Inquiry
CC02502261-03	EzFast Ni FF Prepacked column	5×1mL	Inquiry
CC02502261-04	EzFast Ni FF Prepacked column	5×5mL	Inquiry
CC02502261-05	EzScreen Ni FF Prepacked column	1×4.6mL	Inquiry
CC02502261-06	EzScreen Ni FF Prepacked column	5×4.6mL	Inquiry
CC02502261-07	EzLoad 16/10 Ni FF Prepacked column	1 pcs	Inquiry
CC02502261-08	EzLoad 26/10 Ni FF Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

Ni Bestarose FF (Fast Flow) metal chelation chromatography resin is an affinity chromatography resin that pre-chelates metal ion Ni2+ on agarose gel with NTA as ligand. It has the advantages of large adsorption capacity, good selection type, easy regeneration, and low cost. It is widely used in the separation and purification of downstream proteins and peptides in biopharmaceutical and bioengineering, especially the efficient purification of histidine-tagged proteins.

Specifications

Appearance	Turquoise slurry, can be layered
Matrix	Highly Cross-linked agarose, 6%
Particle size⁺	45~165μm
Ligand concentration	12~18μmol Ligand/mL resin
Dynamic binding capacity	~ 40mg His tag protein/ mL packed resin
Chemical stability ⁺⁺	40°C One week : 10mM HCl, 0.1M NaOH, 6M GuHCl,8M Urea. 40°C 12h: 1M NaOH, 70% acetic acid.
pH stability⁺⁺⁺	3~12 (working) 2~14 (CIP)
Pressure flow velocity	~600cm/h,BXK16/20,H=5cm,25°C
Max. pressure	0.3MPa
Storage⁺⁺⁺⁺	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommend flow velocity	＜150cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ Stability when removing metal ions.
+++ CIP refers to pH stability when metal ions are removed.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.