IMAC Bestarose FF - Tag Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502260-01	EzFast IMAC FF Prepacked column	1×1mL	Inquiry
CC02502260-02	EzFast IMAC FF Prepacked column	1×5mL	Inquiry
CC02502260-03	EzFast IMAC FF Prepacked column	5×1mL	Inquiry
CC02502260-04	EzFast IMAC FF Prepacked column	5×5mL	Inquiry
CC02502260-05	EzScreen IMAC FF Prepacked column	1×4.6mL	Inquiry
CC02502260-06	EzScreen IMAC FF Prepacked column	5×4.6mL	Inquiry
CC02502260-07	EzLoad 16/10 IMAC FF Prepacked column	1 pcs	Inquiry
CC02502260-08	EzLoad 26/10 IMAC FF Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

IMAC Bestarose FF (Fast flow) is a metal chelating affinity chromatography resin, which can be widely used to separate and purify proteins and peptides. The principle is that the side chains of histidine, cysteine and tryptophan of protein interact with a variety of transition metal ions such as Cu2+, Zn2+, Ni2+, Co2+, Fe3+, so as to achieve the purpose of separation and purification.
IMAC Bestarose FF is crosslinked by cross-linked agarose and aminotriacetic acid (NTA). It can chelate the four price points of metal ions, making the chelated metal ions more stable, to tolerate higher reducing agents, have good physical and chemical stability, as well as advantages in good specificity and fast flow velocity.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Particle size⁺	45-165μm
Metal chelating capacity	Ni²⁺/ Zn²⁺:15μmol/mL resin, Cu²⁺:25μmol/mL resin
Dynamic binding capacity	Ni²⁺:~ 40mg His tag protein/mL packed resin
Chemical stability ⁺⁺	1 week at 40°C: 10mM HCl, 0.1M NaOH, 8M urea, 6M GuHCl, 40°C 12h: 1M NaOH, 70% acetic acid.
pH stability⁺⁺⁺	3~12(working),2~14 (CIP)
Pressure flow velocity	~600cm/h（0.1MPa BXK16/20, H=5cm, 25°C）
Max. pressure	0.3MPa
Storage⁺⁺⁺⁺	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	＜150cm/h

+ The particle size is normally distributed, and the particles in this range account for more than 95% of the total.
++ Stability when removing metal ions.
+++ CIP refers to pH stability when metal ions are removed.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.