Benzamidine Bestarose 4FF - Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502266-01	EzFast Benzamidine 4FF Prepacked column	1×1mL	Inquiry
CC02502266-02	EzFast Benzamidine 4FF Prepacked column	1×5mL	Inquiry
CC02502266-03	EzFast Benzamidine 4FF Prepacked column	5×1mL	Inquiry
CC02502266-04	EzFast Benzamidine 4FF Prepacked column	5×5mL	Inquiry
CC02502266-05	EzScreen Benzamidine 4FF Prepacked column	1×4.6mL	Inquiry
CC02502266-06	EzScreen Benzamidine 4FF Prepacked column	5×4.6mL	Inquiry
CC02502266-07	EzLoad 16/10 Benzamidine 4FF Prepacked column	1 pcs	Inquiry
CC02502266-08	EzLoad 26/10 Benzamidine 4FF Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

Benzamidine Bestarose 4FF is an affinity chromatography resin made by coupling p-amino benzamidine to Bestarose 4FF agarose resin. It is commonly used for the separation and purification of serine proteases or the removal of serine proteases from biological samples. The benzamidines are broad-spectrum inhibitors of serine proteases (such as trypsin, thrombin, urokinase, kallikrein, prokinin releasing enzyme, etc) and can be used as ligands for purifying such substances.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 4%
Particle size⁺	45~165μm
Functional group	P-aminobenzidine
Ligand concentration	≥12μmol Ligand/mL resin
Dynamic binding capacity	≥35mg Trypsin/mL packed resin
Max. pressure	0.3MPa
Pressure flow velocity	＞150cm/h（0.1MPa BXK50/30, H=25cm, 20°C）
Chemical stability	Stable in common aqueous buffers: 6M GuHCl,8M Urea, PH =1, 2, 3, 4 hydrochloric acid solution, 0.025M borax solution with pH=8, 9, 10, 11
pH stability	2~8(working),1~9(CIP)
Storage⁺⁺	2~8°C,20% ethanol + 50mM NaAc(pH4.0) or 2% benzyl alcohol + 50mM NaAc(pH 4.0)
Recommended flow velocity	30-300cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.