WiScan Hermes High Content Imaging System

Cat. No.	Description	Specification	Unit	Price	Quantity
LCIS531082-01	Easily generate publication-quality images at high throughput speeds	Bright field: Transmission white LED	Each	Inquiry

Add to Cart

Product Description

Product Description

·Versatile
Whether you are performing assay development, compound screens, transfection assays or looking at a few samples in great detail, and whether you are using 3D models (such as spheroids, Organoids), Zebrafish imaging, primary cells, fixed cells or live cell imaging, WiScan® Hermes is the solution for you.

·Reliable
WiScan Hermes, automated imaging system for high content screening (HCS) provides the unique combination of the two contradicting primary functions of automated microscopy: Image quality and acquisition speed.

·Robust
WiScan® Hermes' mechanisms are based on patents, creatively designed to meet heavy duty operation demands (24/7) with full process robustness.

·Flexible
WiScan® Hermes is a cost-effective system that is both sophisticated and flexible, offering 7 fluorescence colors, bright field option, and a large range of air objectives. The system can accommodate a variety of multi-well plates and sample formats (slides, dishes…) and offers environmental control for live cell assays.

·Intuitive
It doesn't matter if you are a beginner or an experienced microscopist, Hermes easily allows you to look deeper into your samples. The system is intuitively operated. Its built-in applications are extremely easy to use, and are operated at the push-of-a-button.

·Automated immersion media scanning
Now, for the first time, a high-content imaging system can utilize oil immersion objectives for automating high resolution cell biology applications. Samples in a multi-well plate format can be scanned in their entirety, unsupervised and with no user intervention. The system can accommodate a variety of multi-well plates and sample formats and offers environmental control for live cell assays.

Utilizing high NA oil objectives also enables fully automated super-resolution imaging.

Features

Automated immersion media scanning
Now, for the first time, a high-content imaging system can utilize oil immersion objectives for automating high resolution cell biology applications. Samples in a multi-well plate format can be scanned in their entirety, unsupervised and with no user intervention. The system can accommodate a variety of multi-well plates and sample formats and offers environmental control for live cell assays.

Utilizing high NA oil objectives also enables fully automated super-resolution imaging.

Full automation, No Compromises
· Unique hardware automatically adds immersion oil to objectives
· No user intervention; No oil spilling
· Autonomous, rapid image acquisition for:
– Full-plate scanning
– Time-lapse imaging of live cells
· Optimized autofocus, X, Y, Z motion and long-duration oil capsules for easy maintenance

Product Details

Certifications

CE, UL

Specifications

3D reader: EPI-fluorescence inverted optics mounted on XYZ (patented) linear scanner

Auto Focus: Patented ultra-fast laser-based Auto Focus with 100nm resolution
XY motion: Accurate positioning with 200nm repeatability
Illumination sources: Hermes Real-time observer includes 4 fluorescence channels DAPI, GFP, RFP, CY5.
Up to 7 optional LED sources are available in other Hermes models (DAPI, CFP, GFP, YFP, RFP, mCherry, CY5).
Transmission: White LED source
Optical Filters: 2 emission filters and compatible dichroic filters (automatically exchanged)
Objectives (Air): Choice of air objectives in the range: 2X to 60X, high NA.
Oil/ Water immersion objectives: Option to add automated immersion media to allow use of immersion objectives (oil/water) – optional hardware upgrade.
Camera: High sensitivity CMOS camera with 5.1MPixel resolution
Sample format: Supports full-area screening of 6-1536 well plates Supports slides, microarrays, 35 mm dish formats. U-shaped bottom plats are optional. Flexible and simple interface for introducing new sample formats to the microscope.
IT: PC with Windows® operating system and touch screen. Joystick for microscope navigation.
Enclosure: Allows operation in fully lit areas
Desktop standalone platform: 47 W x 72 D x 57 H (cm), 18.5 W x 28.5 D x 22.4 H (inches) With plate cover closed.
Certification: CE, UL

Live cell imaging: Allow long time lapse experiments. Sample does not move during most of the scanning process.
Live cell conditions
Temperature control: Ranges from ambient+5°C to 40°C ±0.5°C
CO2 level control: Enables setting of 0.02 L/min - 0.13 L/min mixed with air for desired CO2 percentage. (external accessory). CO2 Range: 0-15%. Set Point Resolution: 1%
Humidity

Object mapping for rare events detection
First scan: rapid scan of entire plate with low magnification, for efficient region of interest object mapping.
Second scan: re-visitng solely detected objects with high magnification
Analysis tools: WiSoft® based image processing tools for object definition and detection in the first low magnification scan and further tools to analyze the detected objects with high magnification
Objectives: Automatic objective exchanger, accommodates of up to 3 objectives at a time
Throughput: Enhances the throughput of high magnification scanning of rare objects
Statistical tools: Provides automatic decision resulting in efficient screening for minimum required objects in a well
Combination of HCS and HTS: Ultra-fast High Content Screening unit
Image acquisition speed >10 images per second (depends on experiment conditions)
Throughput / Acquisition speed: A full 96-well plate screening using 10X magnification with a single field per well, four fluorescence colors, 50ms exposure time per channel, runs in ~2 minutes. With some applications, this will include data processing and analysis results simultaneously with image acquisition.
Automation Remote accessibility: Protocols (applications and screening parameters) execution and management
External loader compatibility to 3rd-party robotics (Robot/manipulator)
Communication channel for control by external equipment
Loading door with automatic opening/closing synchronized with external equipment
External loader (Robot/manipulator) integration: Full integration supported

DAPI/HOCHEST: Ex.: 390/18; Em.: 440/40
CFP: Ex.: 435/40; Em.: 482/35
FITC/GFP: Ex.: 482/25; Em.: 525/30
YFP: Ex.: 510/20; Em.: 542/27
TRITC/RFP/Cy3: Ex.: 559/34; Em.: 607/36
mCherry: Ex.: 580/23; Em.: 624/40
Cy5: Ex.: 650/13; Em.: 694/44

Hermes system incorporates an automated objective exchanger which holds up to 3 objectives at a time.

Hermes supports air objectives and can optionally support a unique immersion media dispensing to allow oil/water immersion objectives.

Objectives selection:

Magnification	N.A.	µm/pixel	Working Distance [µm]	Imm. Media
2X	0.08	3.0	6200	Air
4X	0.16	1.5	13000	Air
10X	0.40	0.6	3100	Air
20X	0.45	0.3	6600-7800	Air
20X	0.80	0.3	600	Air
40X	0.60	0.15	2700-4000	Air
40X	0.75	0.15	510	Air
40X	0.90	0.15	200	Air
60X	0.95	0.1	200	Air
40X	1.4	0.15	130	Oil
60X	1.42	0.1	150	Oil
100X	1.45	0.065	130	Oil
20X	0.7	0.3	350	Water
40X	1.15	0.15	250	Water
60X	1.2	0.1	280	Water