SP Bestarose HP - Strong Cation Exchange Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502296-01	EzFast SP HP Prepacked column	1×1mL	Inquiry
CC02502296-02	EzFast SP HP Prepacked column	5×1mL	Inquiry
CC02502296-03	EzFast SP HP Prepacked column	1×5mL	Inquiry
CC02502296-04	EzFast SP HP Prepacked column	5×5mL	Inquiry
CC02502296-05	EzScreen SP HP Prepacked column	1×4.6mL	Inquiry
CC02502296-06	EzScreen SP HP Prepacked column	5×4.6mL	Inquiry
CC02502296-07	EzLoad 16/10 SP HP Prepacked column	1 pcs	Inquiry
CC02502296-08	EzLoad 26/10 SP HP Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
SP Bestarose HP (high performance) is a strong cation exchange resin formed by coupling sulfopropyl groups on fine-grained high-resolution agarose microspheres.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Functional group	Sulfopropyl
Particle size+	24~44μm (the average particle size is 34μm)
Ionic capacity	150~200μmol H⁺/mL packed resin
Dynamic binding capacity ++	~ 70 mg BSA/mL resin
Max. pressure	0.3MPa
Chemical Stability	Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++,6M GuHCl, 30%isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents.
pH stability	3~14(CIP), 4~13(working)
Operating temperature	Operating temperature:2~40°C, do not freeze.
Storage++++	2~30°C, 20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc
Recommended flow velocity	60-150cm/h

+ Average particle size is the accumulated resin particle size of packing volume distribution.
++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load at 100mM NaHAc pH9 flow velocity of 1mL /min,
+++1M NaOH and 1M HAc only be used for cleaning.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.