SP Bestarose FF - Strong Cation Exchange Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502298-01	EzFast SP FF Prepacked column	1×1mL	Inquiry
CC02502298-02	EzFast SP FF Prepacked column	5×1mL	Inquiry
CC02502298-03	EzFast SP FF Prepacked column	1×5mL	Inquiry
CC02502298-04	EzFast SP FF Prepacked column	5×5mL	Inquiry
CC02502298-05	EzScreen SP FF Prepacked column	1×4.6mL	Inquiry
CC02502298-06	EzScreen SP FF Prepacked column	5×4.6mL	Inquiry
CC02502298-07	EzLoad 16/10 SP FF Prepacked column	1 pcs	Inquiry
CC02502298-08	EzLoad 26/10 SP FF Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
SP Bestarose FF (Fast Flow) is a strong cation exchanger formed by coupling sulfopropyl onto high flow velocity agarose matrix.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Functional group	Sulfopropyl
Particle size⁺	45~165μm
Ionic capacity	180-250μmol/ mL packed resin
Max. pressure	0.3MPa
Pressure flow velocity⁺⁺	≥400cm/h（0.1MPa,BXK50/30,H=15cm,20°C,0.1M NaCl）
Chemical Stability	Stable in common aqueous buffers:1M NaOH⁺⁺⁺,1M HAC⁺⁺⁺,10mM NaOH, 6M GuHCl,8M Urea, 70% ethanol ,30% Isopropyl alcohol, 20% ethanol, 2%Benzyl alcohol, 30% acetonitrile
pH stability	3 ~14 (CIP), 4~13(Working)
Operating temperature	Operating temperature:2~40°C, do not freeze.
Storage++++	2~30°C, 20% ethanol +0.2M NaAc or 2% benzyl alcohol +0.2M NaAc
Recommend flow velocity	60-300cm/h
Avoid	Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20°C) to pH <4.

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++1M NaOH and 1M HAc only be used for cleaning.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.