MMC Bestarose FF - MMC Chromatography Columns

Cat. No. Specification Unit Price Quantity
CC02502299-01 EzFast MMC FF Prepacked column 1×1mL Inquiry
CC02502299-02 EzFast MMC FF Prepacked column 5×1mL Inquiry
CC02502299-03 EzFast MMC FF Prepacked column 1×5mL Inquiry
CC02502299-04 EzFast MMC FF Prepacked column 5×5mL Inquiry
CC02502299-05 EzScreen MMC FF Prepacked column 1×4.6mL Inquiry
CC02502299-06 EzScreen MMC FF Prepacked column 5×4.6mL Inquiry
CC02502299-07 EzLoad 16/10 MMC FF Prepacked column 1 pcs Inquiry
CC02502299-08 EzLoad 26/10 MMC FF Prepacked column 1 pcs Inquiry

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Product Description
Product Description
Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
MMC Bestarose FF (Fast Flow) is a mixed-mode weak cation exchange resin formed by coupling complex groups with benzene ring and carboxyl group on agarose microspheres with high Flow velocity.
Specifications

Appearance

White slurry

Matrix

Cross-linked agarose, 6%

Functional group

Complex weak cationic groups

Average particle size

~90μm

Ionic capacity

90~120μmol H+/mL packed resin

Max. pressure

0.3MPa

Pressure flow velocity+

≥700cm/h(0.1MPa, BXK 50/30, H=15cm, 20°C)

Chemical Stability

Stable in common aqueous buffers:1M NaOH++,1M HAC++,6M GuHCl,8M Urea, 70% ethanol ,20% ethanol

Avoid contact with oxidizing agents, cationic detergents.

pH stability

2~14 (CIP), 4~13(working)

Operating temperature

Operating temperature:2~40°C, do not freeze.

Storage+++

2~30°C, 20% ethanol or 2% benzyl alcohol

Recommend flow velocity

60-300cm/h

+ This flow velocity is a linear flow velocity of BXK50/30 column tube with column bed height of 15cm and pressure of 0.1MPa at 20°C. Pressure flow velocity is the relationship between pressure and flow velocity at a particular pressure, and the pressure given should not be regarded as the maximum tolerated pressure of the resin.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
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