Q Bestarose XL - Strong Anion Exchange Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502291-01	EzFast Q XL Prepacked column	1×1mL	Inquiry
CC02502291-02	EzFast Q XL Prepacked column	5×1mL	Inquiry
CC02502291-03	EzFast Q XL Prepacked column	1×5mL	Inquiry
CC02502291-04	EzFast Q XL Prepacked column	5×5mL	Inquiry
CC02502291-05	EzScreen Q XL Prepacked column	1×4.6mL	Inquiry
CC02502291-06	EzScreen Q XL Prepacked column	5×4.6mL	Inquiry
CC02502291-07	EzLoad 16/10 Q XL Prepacked column	1 pcs	Inquiry
CC02502291-08	EzLoad 26/10 Q XL Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Bestarose XL series resin is obtained by coupling linear dextran molecules on highly cross-linked agarose matrix, before coupling various functional groups to dextran molecules. This structure reduces the steric hindrance effect among biological molecules, greatly improving the binding of the target molecule.
Q Bestarose XL is a strong anion exchange resin, which can directly capture biological macromolecules such as viruses from the feed stock. It enjoys high binding capacity and easy scale-up for industrial processing.

Specifications

Appearance	White slurry
Matrix	6% highly cross-linked agarose with dextran chain
Functional group	Quaternary amine group
Particle size+	45~165μm
Ionic capacity	180~260μmol Cl-/mL packed resin
Dynamic binding capacity++	> 160 mg BSA/mL resin
Max. pressure	0.3MPa
Pressure flow velocity+++	≥700cm/h (0.1MPa, BXK 50/30 ,H=15cm, 20°C)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++++, 6M GuHcl, 30% isopropyl alcohol, 20% ethanol Avoid contact with oxidizing agents.
pH stability	2~14(CIP), 2~12(working)
Operating temperature	Operating temperature:2~40°C, do not freeze. Can tolerate 121°C autoclaving (20min) .
Storage+++++	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	300~500cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ In EzScreen 4.4mL column with column height of 10cm, 10%BSA dynamic binding load under 50mM Tris+1M NaCl pH 7.5, flow velocity of 2.2mL /min.
+++The pressure flow velocity is linear flow velocity of BXK50/30 column, column bed height 15cm, 20°C 0.1MPa pressure. Pressure flow velocity is the relationship between pressure and flow velocity at a particular pressure, ++and the pressure given should not be regarded as the maximum tolerated pressure of the resin.
++++1M NaOH only be used for cleaning.
+++++2% benzyl alcohol is only used for international transport or special requirements from customer.