Q Bestarose FF - Strong Anion Exchange Chromatography Columns

Cat. No. Specification Unit Price Quantity
CC02502292-01 EzFast Q FF Prepacked column 1×1mL Inquiry
CC02502292-02 EzFast Q FF Prepacked column 5×1mL Inquiry
CC02502292-03 EzFast Q FF Prepacked column 1×5mL Inquiry
CC02502292-04 EzFast Q FF Prepacked column 5×5mL Inquiry
CC02502292-05 EzScreen Q FF Prepacked column 1×4.6mL Inquiry
CC02502292-06 EzScreen Q FF Prepacked column 5×4.6mL Inquiry
CC02502292-07 EzLoad 16/10 Q FF Prepacked column 1 pcs Inquiry
CC02502292-08 EzLoad 26/10 Q FF Prepacked column 1 pcs Inquiry

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Product Description
Product Description
Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Q Bestarose FF (Fast Flow) is a strong cation exchanger formed by coupling quaternary amine group onto high flow velocity agarose matrix.
Specifications

Appearance

White slurry

Matrix

Cross-linked agarose, 6%

Functional group

Quaternary amine group

Particle size+

45~165μm

Ionic capacity

180-240μmol Cl-/mL packed resin

Max. pressure

0.3MPa

Pressure flow velocity++

≥400cm/h(0.1MPa,BXK50/30,H=15cm ,20°C,0.1 M NaCl)

Chemical Stability

Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++,6M GuHCl,8M Urea, 70% ethanol , 30% Isopropyl alcohol, 20% ethanol, 2%Benzyl alcohol

pH stability

2 ~14 (CIP), 2~12 (Working)

Operating temperature

Operating temperature:2~40°C, do not freeze.

Storage++++

2~30°C, 20% ethanol or 2% benzyl alcohol.

Recommend flow velocity

60-300cm/h

Avoid

Avoid contact with oxidizing agents, cationic detergents.

Avoid long time exposures (1 week, 20°C) to pH <4.

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++1M NaOH and 1M HAc only be used for cleaning.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.
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