SP Bestarose BB - Strong Cation Exchange Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502293-01	EzFast SP BB Prepacked column	1×1mL	Inquiry
CC02502293-02	EzFast SP BB Prepacked column	5×1mL	Inquiry
CC02502293-03	EzFast SP BB Prepacked column	1×5mL	Inquiry
CC02502293-04	EzFast SP BB Prepacked column	5×5mL	Inquiry
CC02502293-05	EzScreen SP BB Prepacked column	1×4.6mL	Inquiry
CC02502293-06	EzScreen SP BB Prepacked column	5×4.6mL	Inquiry
CC02502293-07	EzLoad 16/10 SP BB Prepacked column	1 pcs	Inquiry
CC02502293-08	EzLoad 26/10 SP BB Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin.
(3) An ion (called an equilibrium ion) has an opposite charge to the functional group and can be reversibly bound to the functional group.
Big Beads (BB) resin is based on highly cross-linked big beads agarose base frame. The large particle can tolerate samples with poor clarity, reducing the possibility of column blockage. Besides, its fast flow velocity is suitable for processing large samples in a short time.
SP Bestarose BB is a strong cation exchange resin that can capture biological macromolecules directly from the feed stock. Its high binding capacity and fast flow velocity make it suitable for large-scale production applications.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Functional group	Sulfopropyl
Particle size⁺	100~300μm
Ionic capacity	180~250μmol H⁺/mL packed resin
The exclusion limit, Mr	~4× 10⁶ (Globular protein)
Max. pressure	0.3MPa
Pressure flow velocity⁺⁺	≥1200cm/h（0.1MPa BXK50/30, H=15cm, 20°C, 0.1M NaCl）
Chemical stability	Stable in common aqueous buffers: 1M NaOH+++,6M GuHCl,30% isopropyl alcohol,70% ethanol Avoid contact with oxidizing agents
pH stability	3~14(CIP), 4~13 (working)
Operating temperature	Operating temperature:2~40°C, do not freeze.
Storage++++	2~30°C, 20% ethanol + 0.2M NaAc or 2% benzyl alcohol + 0.2M NaAc
Recommended flow velocity	200~600cm/h

+Particle size is normally distributed, and particles within this range account for more than 80% of the total l.
++ The pressure/flow characteristics describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++1M NaOH only be used for cleaning.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.