Novo-A Diamond - Protein A Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502246-01	EzFast Novo-A Diamond Prepacked column	1×1mL	Inquiry
CC02502246-02	EzFast Novo-A Diamond Prepacked column	1×5mL	Inquiry
CC02502246-03	EzFast Novo-A Diamond Prepacked column	5×1mL	Inquiry
CC02502246-04	EzFast Novo-A Diamond Prepacked column	5×5mL	Inquiry
CC02502246-05	EzScreen Novo-A Diamond Prepacked column	1×4.6mL	Inquiry
CC02502246-06	EzScreen Novo-A Diamond Prepacked column	5×4.6mL	Inquiry
CC02502246-07	EzSelect Extrem A Diamond Prepacked column	1×600μL	Inquiry
CC02502246-08	EzLoad 16/10 Novo-A Diamond Prepacked column	1 pcs	Inquiry
CC02502246-09	EzLoad 26/10 Novo-A Diamond Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Novo-A Diamond is a new generation of enhanced alkali-resistant resin independently developed by Bestchrom. The protein A ligand is expressed in E. coli. Compared with previous AT Protein A Diamond Plus, it enjoys higher tolerance towards alkali (can tolerate CIP with 0.5-1.0M NaOH) and higher binding capacity. They can effectively cut cost during scale-up by choosing lower column volume. Besides, they are suitable for capturing monoclonal antibodies or Fc fusion protein from large volume cell culture media, as well as the capture of polyclonal antibodies from ascites or plasma.
The resin has the following characteristics:
▪ Under the same residence time, it has higher dynamic binding capacity than conventional products.
▪ The high alkali tolerance of protein ligands can withstand CIP of 0.5~1.0M NaOH during the cleaning process.
▪ High binding capacity, can reduce production cost and improve production efficiency.

Specifications

Appearance	White slurry
Matrix	High rigidity agarose
Average particle size+	~75μm
Functional group	Recombinant Alkali Tolerant Protein A
Cross-linking method	Epoxy chemistry
Dynamic binding capacity++	≥65mg Human IgG/mL packed resin (Residence time 6min)
Chemical stability	Stable in common aqueous buffers: 10mM HCl, 0.1Msodium citrate (pH3), 6M GuHCl, 8M Urea, 30% Isopropyl alcohol, 20% ethanol.
Max. pressure	0.5MPa
Pressure flow velocity	≥500cm/h,<2bar,BXK300/500,H=20cm
pH stability	3~12(working) 2~14(CIP)
Storage+++	2~8°C, 20% ethanol or 2% benzyl alcohol

+Particle size is the cumulative resin particle size of packing volume distribution.
++Linear flow velocity 100cm / h, column height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.