Extrem A Diamond - Protein A Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502248-01	EzFast Extrem A Diamond Prepacked column	1×1mL	Inquiry
CC02502248-02	EzFast Extrem A Diamond Prepacked column	1×5mL	Inquiry
CC02502248-03	EzFast Extrem A Diamond Prepacked column	5×1mL	Inquiry
CC02502248-04	EzFast Extrem A Diamond Prepacked column	5×5mL	Inquiry
CC02502248-05	EzScreen Extrem A Diamond Prepacked column	1×4.6mL	Inquiry
CC02502248-06	EzScreen Extrem A Diamond Prepacked column	5×4.6mL	Inquiry
CC02502248-07	EzSelect Extrem A Diamond Prepacked column	1×600μL	Inquiry
CC02502248-08	EzLoad 16/10 Extrem A Diamond Prepacked column	1 pcs	Inquiry
CC02502248-09	EzLoad 26/10 Extrem A Diamond Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Extrem A Diamond is an innovative enhanced alkali-resistant resin independently developed by Bestchrom. The protein A ligand is expressed in E. coli. Compared with previous AT Protein A Diamond Plus, it enjoys higher tolerance towards alkali (can tolerate CIP with 0.5-1.0M NaOH) and higher binding capacity. It can effectively cut cost during scale-up by choosing lower column volume. Besides, It is suitable for capturing monoclonal antibodies or Fc fusion protein from large volume cell culture media, as well as the capture of polyclonal antibodies from ascites or plasma.
The resin has the following characteristics:
▪ Under the same residence time, it has higher dynamic binding capacity than conventional products.
▪ The high alkaline tolerance of protein ligands can withstand CIP of 0.5~1.0M NaOH during the cleaning process.
▪ High binding capacity, reduced production cost and improved production efficiency.

Specifications

Appearance	White slurry
Matrix	High rigidity agarose
Average particle size +	~60μm
Functional group	Recombinant Alkali Tolerant Protein A
Cross-linking method	Epoxy chemistry
Dynamic binding capacity ++	≥75mg Human IgG/mL packed resin(Residence time 6min)
Chemical stability	Stable in common aqueous buffers: 10mM HCl, 0.1Msodium citrate, 6M GuHCl, 8M Urea, 30% Isopropyl alcohol, 20% ethanol.
Max. pressure	0.5MPa
Pressure flow velocity	≥300cm/h,<2bar,BXK300/500,H=20cm
pH stability	3~12(working) 2~14(CIP)
Storage+++	2~8°C, 20% ethanol or 2% benzyl alcohol

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++Linear flow velocity 100cm / h, column height 10cm, buffer conditions: 20mM PB, 0.15M NaCl, pH7.4.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.