AT Protein A Diamond - Protein A Affinity Chromatography Columns

AT Protein A Diamond is an innovative affinity chromatography resin made by coupling alkali-resistant Protein A with high rigidity agarose matrix through epoxy activation. Compared with traditional antibody affinity resin, the ligand enjoys better stability, faster flow velocity and lower back pressure. The resin is suitable for capturing monoclonal antibodies or Fc fusion proteins from mass cell culture media, as well as polyclonal antibodies from ascites or plasma.
The ligand of AT Protein A Diamond is the fragment of recombinant Protein A obtained by fermentation of E.Coli. During upstream construction, alkali-resistant amino acids were used to replace the non-alkali-resistant amino acids, while protease-sensitive amino acids were removed, providing the ligand with good alkali tolerance. The ligand fermentation and subsequent purification processes use no animal derived raw materials. The resin can tolerate CIP with 0.1~0.5M NaOH, avoiding the use of expensive and corrosive cleaning reagents, which effectively cuts costs and simplifies industrial-scale production.