Diamond Blue - Affinity Chromatography Columns

Cat. No. Specification Unit Price Quantity
CC02502264-01 EzFast Diamond Blue Prepacked column 1×1mL Inquiry
CC02502264-02 EzFast Diamond Blue Prepacked column 1×5mL Inquiry
CC02502264-03 EzFast Diamond Blue Prepacked column 5×1mL Inquiry
CC02502264-04 EzFast Diamond Blue Prepacked column 5×5mL Inquiry
CC02502264-05 EzScreen Diamond Blue Prepacked column 1×4.6mL Inquiry
CC02502264-06 EzScreen Diamond Blue Prepacked column 5×4.6mL Inquiry
CC02502264-07 EzLoad 16/10 Diamond Blue Prepacked column 1 pcs Inquiry
CC02502264-08 EzLoad 26/10 Diamond Blue Prepacked column 1 pcs Inquiry

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Product Description
Product Description
Diamond Blue is a kind of affinity resin based on high rigidity agarose with fine particles. The ligand is Cibacron Blue 3GA, which is physically and chemically stable. Since the ligand is not easy to drop off, it can enjoy long service life and a wide range of applications. The resin can bind proteins through specific interactions, as well as non-specific binding through electric charges. Diamond Blue has been widely used in the separation and purification of various proteins, including dehydrogenase, kinase, transferase, serum albumin, interferon and plasma proteins.
Compared to Blue Bestarose FF, Diamond Blue has better pressure flow velocity performance and higher binding capacity, helping to improve process efficiency and reduce cost.
Specifications

Appearance

Blue slurry

Matrix

High cross-linked rigidity agarose

Average particle size +

~75μm

Functional group

Cibacron Blue 3GA

Ligand concentration

~13μmol chromophore /mL resin

Dynamic binding capacity++

≥24mg HSA/mL packed resin

Chemical Stability

40°C 1 week:10mM HCl,0.1M NaOH, 8M urea, 6M GuHCl

40°C 12h:1M NaOH, 70% acetic acid

Max. pressure

0.5MPa

Pressure flow velocity

≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20°C)

pH stability

2~13(working),2~13(CIP)

Temperature tolerance

Working temperature:2~40°C, Can't freeze.

Storage+++

2~8°C,20% ethanol + 0.1M KH2PO4(pH 8.0)or 2% benzyl alcohol + 0.1M KH2PO4(pH 8.0)

+ Average particle size is the accumulated resin particle size of packing volume distribution.
++ Residence time: 4min.
+++2% benzyl alcohol is only used for international transport or special requirements from customer. + Average particle size is the accumulated resin particle size of packing volume distribution.
++ Residence time: 4min.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.
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