Plasmid Cap Bestarose HP - Affinity Chromatography Columns

Cat. No. Specification Unit Price Quantity
CC02502271-01 EzFast Plasmid HP Prepacked column 1×1mL Inquiry
CC02502271-02 EzFast Plasmid HP Prepacked column 1×5mL Inquiry
CC02502271-03 EzFast Plasmid HP Prepacked column 5×1mL Inquiry
CC02502271-04 EzFast Plasmid HP Prepacked column 5×5mL Inquiry
CC02502271-05 EzScreen Plasmid HP Prepacked column 1×4.6mL Inquiry
CC02502271-06 EzScreen Plasmid HP Prepacked column 5×4.6mL Inquiry
CC02502271-07 EzLoad 16/10 Plasmid HP Prepacked column 1 pcs Inquiry
CC02502271-08 EzLoad 26/10 Plasmid HP Prepacked column 1 pcs Inquiry

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Product Description
Product Description
Plasmid Cap Bestarose HP is a thiophilic affinity resin made by fixing sulfur-containing compound 2-mercapyridine on high resolution crosslinked agratose. Its optimized ligand density has appropriate affinity with superhelix DNA, and fine particle microspheres can improve the binding capacity of superhelix DNA with larger molecular weight. The principle of thiophilic affinity is to use the interaction between electron donor and electron acceptor to separate and purify biomolecules. This force is strengthened in high salt environment and weakened in low salt environment.
Specifications

Appearance

White slurry

Matrix

Highly Cross-linked agarose, 6%

Average particle size

~34μm

Functional group

2-mercaptopyridine

Ligand concentration

~3.5mg 2-mercaptopyridine /mL resin

Dynamic binding capacity

>2mg Supercoiled plasmid DNA /mL packed resin

Chemical Stability

Stable in common aqueous buffers: 1M HAC+ ,30% isopropyl alcohol, 70% ethanol , 0.1M NaOH

Max. pressure

0.3MPa

pH stability

3~11(Working), 2~13(CIP)

Storage++

2~30°C, 20% ethanol or 2% benzyl alcohol

Operating temperature

Operating temperature:15~30°C

+1M 1M HAc only be used for cleaning.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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