GER Wave-Mixed Bioreactor (10L-50L)

GER series wave bioreactor is an efficient cell culture system,with optional sensor modules and the automated control software system, and paired with the GER single-use wave-mixed bioreactor bags. The working volume can reach 25L, providing solutions for general life science research in the biopharmaceutical field, seed tank cultivation, and cGMP production.

Configuration of Wave-mixed Bioreactor

Waving Module
• Pendulum swings blends surface gas into culture solution quickly and fully;
• Allowing the cells, gas and nutrients in the bioculture bag to be uniformly distributed; adopting the heating film mode to maintain stable and uniform temperature-rise period.

pH Control Module
pH value in the process of real-time control
• Automatic mode: pH sensor is associated with the acid- base pump and CO2 to achieve closed-loop control. The cumulative acid-base flow is recorded.
• Manual mode: CO2 gas valve, acid pump and alkali pump are supported to be independently controlled on the human- machine interface

DO Control Module
• Automatic Mode: DO value is real-time controlled in the process. DO sensor is connected to Air gas valve, N2 gas valve and O2 gas valve to achieve closed-loop control and form the cumulative system. The real-time flow of N2 and O2 is recorded, and the integrated value is counted.
• Manual Mode: Air, N2 and O2 are supported to be independently controlled on computer. The system records the real-time flow and cumulative value of AIR, N2 and O2.

Software System
• The system supports the access control, user management, electronic signature and recording, audit tracking and change control, data archiving, curve analysis and data backup, alarm prompt
• The system is prepared for GMP needs .Compliance with GAMPS, FDA and 21 CFR Part 11, as well as other relevant laws and regulations

Perfusion Control Unit (Optional)
• Perfusion culture is achieved by coupling a peristaltic pump with weight feedback, combined with a retention membrane on the bag

Weighing Module
• Real-time detection of cell weight change value in the process.

Excellent Cultivation Performance
• Rapid mixing and improvement of gas mass transfer
• No need of bubble ventilation during oxygen mass transfer to avoid cell damage and the use of defoaming agent
• Improving cell status and enhancing density and yield

Flexible Configuration and Convenient Operation
• Compatible with reaction bags of different specifications,which results in the hardware input cost reduction
• Reduction of vessel contents transfer, lowering the pollution risks and saving working time
• Avoiding cross contamination, sterilization by washing, and cleaning validation

Powerful Platform Universality
• Suitable for culture of most suspension cells and flaky carrier
• Compatible with culture modes including batch culture, batch feeding and perfusion

Accurate control system
• Accurate control of pendulum angle and rotation speed;
• Working with pH+DO sensors to offer the measurement of key process parameters;
• Omnidirectional monitoring of cell culture process;

Used in the production of biopharmaceuticals, vaccines, gene therapy,and other products, typical applications include:
• Suspension culture of mammalian cells, such as CHO or HEK293 cells
• Microcarrier culture of adherent cells, such as Vero
• Insect cell culture, such as Sf9 cells
• Immune cells, such as stem cells, and continuous perfusion of T cells
• Batch and fed batch culture of suspension cells