Diamond Protein L - Antibody Affinity Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502253-01	EzFast Diamond Protein L Prepacked column	1×1mL	Inquiry
CC02502253-02	EzFast Diamond Protein L Prepacked column	1×5mL	Inquiry
CC02502253-03	EzFast Diamond Protein L Prepacked column	5×1mL	Inquiry
CC02502253-04	EzFast Diamond Protein L Prepacked column	5×5mL	Inquiry
CC02502253-05	EzScreen Diamond Protein L Prepacked column	1×4.6mL	Inquiry
CC02502253-06	EzScreen Diamond Protein L Prepacked column	5×4.6mL	Inquiry
CC02502253-07	EzSelect Diamond Protein L Prepacked column	1×600μL	Inquiry
CC02502253-08	EzLoad 16/10 Diamond Protein L Prepacked column	1 pcs	Inquiry
CC02502253-09	EzLoad 26/10 Diamond Protein L Prepacked column	1 Pcs	Inquiry

Product Description

Product Description

Diamond Protein L is an innovative affinity resin obtained by coupling Protein L ligands on the highly rigid agarose base matrix via epoxy activation. Its high rigidity agarose enjoys higher flow velocity and lower back pressure than conventional resins. Besides, the variable zone between the Protein L ligands and Kappa antibody light chain possesses strong affinity. It is also suitable for the capture of antibody fragments, including Fab, single chain antibody fragment (scFv) and regional antibody (dAbs), from large-volume cell culture medium.
Advantages:
▪ High rigid agarose base matrix, enabling high flow velocity and improved productivity.
▪ Highly specific Kappa light chain, enabling efficient capture of antibody and antibody fragments with wide selection.
▪ High binding capacity, reduce production time and resin consumption, lower cost and improved productivity.

Specifications

Appearance	White slurry
Matrix	Highly rigid agarose
Average particle size⁺	~80μm
Functional group	Recombinant Protein L (from E.coli)
Cross-linking method	Epoxy chemistry
Dynamic binding capacity⁺⁺	~40 mg Human IgG/mL packed resin(Retention time 6min)
Chemical stability	Common aqueous solutions: 10mM HCl,0.1M citric acid(pH3),6M Urea, 6M GuHCl, 30% isopropanol, 20%ethanol
Max. pressure	0.5MPa
Pressure flow velocity	≥300cm/h,<2bar,BXK300/500, H=20cm
pH stability	2~10(working), 15mM NaOH (CIP)
Storage⁺⁺⁺	2~8°C, 20% ethanol or 2% benzyl alcohol

+ Particle size is normally distributed, average particle size is the medium value of the particle size in resin.
++Flow velocity 100cm/h, bed height 10cm, Buffer solution: 20mM PB, 0.15M NaCl, pH7.2.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.