Diamond Phenyl(LS) - Phenyl HIC Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502306-01	EzFast Diamond Phenyl(LS) Prepacked column	1×1mL	Inquiry
CC02502306-02	EzFast Diamond Phenyl(LS) Prepacked column	5×1mL	Inquiry
CC02502306-03	EzFast Diamond Phenyl(LS) Prepacked column	1×5mL	Inquiry
CC02502306-04	EzFast Diamond Phenyl(LS) Prepacked column	5×5mL	Inquiry
CC02502306-05	EzScreen Diamond Phenyl(LS) Prepacked column	1×4.6mL	Inquiry
CC02502306-06	EzScreen Diamond Phenyl(LS) Prepacked column	5×4.6mL	Inquiry
CC02502306-07	EzLoad 16/10 Diamond Phenyl(LS) Prepacked column	1 pcs	Inquiry
CC02502306-08	EzLoad 26/10 Diamond Phenyl(LS) Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Diamond Phenyl (LS) is based on high-rigidity agarose with low substitution and strong hydrophobic phenyl groups. It has low back pressure and fast flow velocity, which is suitable for separation and purification of biomolecules on large scale.

Specifications

Appearance	White slurry
Matrix	High rigidity agarose
Functional group	Phenyl
Particle size⁺	40~120μm
Ligand concentration	~ 22μmol Ligand/mL resin
Dynamic binding capacity	~22 mg BSA/mL packed resin
Max. pressure	0.5MPa
Pressure flow velocity	≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20°C)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C autoclaving (20min)
Storage+++	2~30°C,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~350cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.