Phenyl Bestarose HP - Phenyl HIC Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502307-01	EzFast Phenyl HP Prepacked column	1×1mL	Inquiry
CC02502307-02	EzFast Phenyl HP Prepacked column	5×1mL	Inquiry
CC02502307-03	EzFast Phenyl HP Prepacked column	1×5mL	Inquiry
CC02502307-04	EzFast Phenyl HP Prepacked column	5×5mL	Inquiry
CC02502307-05	EzScreen Phenyl HP Prepacked column	1×4.6mL	Inquiry
CC02502307-06	EzScreen Phenyl HP Prepacked column	5×4.6mL	Inquiry
CC02502307-07	EzLoad 16/10 Phenyl HP Prepacked column	1 pcs	Inquiry
CC02502307-08	EzLoad 26/10 Phenyl HP Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Phenyl Bestarose HP is based on highly cross-linked agarose, which retains excellent hydrophilicity and pore structure of natural polysaccharide compounds while having good compatibility with biological macromolecules. The average particle size of Phenyl Bestarose HP is 34μm, which enjoys higher resolution efficiency than Phenyl Bestarose FF. The surface contains Phenyl hydrophobic ligands, which are particularly suitable for the polishing and purification of recombinant proteins, antibodies, vaccines, VLP particles and other highly hydrophobic biological molecules.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Particle size+	24~44μm(the average particle size is 34μm)
Functional group	Phenyl
Ligand concentration	~ 25μmol Ligand/mL resin
Dynamic binding capacity	~ 45mg α-Chymotrypsinogen /mL packed resin
Max. pressure	0.3MPa
Pressure flow velocity	≥200cm/h(0.1MPa,BXK 50/30, H=15cm, 25°C)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++, 1M HAc++, 6M GuHCl, 30% isopropyl alcohol, 70% ethanol
pH stability	3~13(CIP), 3~12(working)
Operating temperature	Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C high pressure sterilization (20min)
Storage+++	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	60-120cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.