Diamond Phenyl Mustang - Phenyl HIC Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502308-01	EzFast Phenyl Mustang Prepacked column	1×1mL	Inquiry
CC02502308-02	EzFast Phenyl Mustang Prepacked column	5×1mL	Inquiry
CC02502308-03	EzFast Phenyl Mustang Prepacked column	1×5mL	Inquiry
CC02502308-04	EzFast Phenyl Mustang Prepacked column	5×5mL	Inquiry
CC02502308-05	EzScreen Phenyl Mustang Prepacked column	1×4.6mL	Inquiry
CC02502308-06	EzScreen Phenyl Mustang Prepacked column	5×4.6mL	Inquiry
CC02502308-07	EzLoad 16/10 Phenyl Mustang Prepacked column	1 pcs	Inquiry
CC02502308-08	EzLoad 26/10 Phenyl Mustang Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Diamond Phenyl Mustang is based on fine particle high-rigidity agarose with a hydrophobic phenyl group. The high resolution and low back pressure are suitable for the intermediate purification stage and fine purification stage of protein downstream purification.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked agarose
Functional group	Phenyl
Average particle size	36~44μm
Hydrophobicity	The retention time of Lyzosyme is 45-50min
Dynamic binding capacity	~19mg BSA/mL packed resin
Max. pressure	0.5MPa
Pressure flow velocity	≥450cm/h(0.5MPa BXK 100/500, H=20cm, 20°C)
Chemical stability	Stable in common aqueous buffers: 2M NaOH+,0.5M NaOH+,1M HAc+, 6M GuHCl,8M Urea,30% isopropyl alcohol,30% acetonitrile,2%SDS,3M(NH₄)₂SO₄+ 0.1M KH₂PO₄(pH7.0)
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C high pressure sterilization (20min)
Storage++	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	90~180cm/h

+1M NaOH and 1M HAc only be used for cleaning.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.