Diamond Octyl - Octyl HIC Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502312-01	EzFast Diamond Octyl Prepacked column	1×1mL	Inquiry
CC02502312-02	EzFast Diamond Octyl Prepacked column	5×1mL	Inquiry
CC02502312-03	EzFast Diamond Octyl Prepacked column	1×5mL	Inquiry
CC02502312-04	EzFast Diamond Octyl Prepacked column	5×5mL	Inquiry
CC02502312-05	EzScreen Diamond Octyl Prepacked column	1×4.6mL	Inquiry
CC02502312-06	EzScreen Diamond Octyl Prepacked column	5×4.6mL	Inquiry
CC02502312-07	EzLoad 16/10 Diamond Octyl Prepacked column	1 pcs	Inquiry
CC02502312-08	EzLoad 26/10 Diamond Octyl Prepacked column	1 pcs	Inquiry

Add to Cart

Product Description

Product Description

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Diamond Octyl is obtained by coupling moderately hydrophobic aliphatic octyl groups to high-rigidity agarose matrix. It enjoys low backpressure and fast flow velocity, which is suitable for large-scale purification of biomolecules on large scale.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked rigid agarose
Functional group	Octyl
Ligand concentration	~5μmol Ligand/mL resin
Particle size⁺	40~120μm
Max. pressure	0.5MPa
Pressure flow velocity	≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20°C)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP),3~13 (working)
Operating temperature	Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C autoclaving (20min)
Storage+++	2~30°C,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~350cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.