Octyl Bestarose 4FF - Octyl HIC Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502313-01	EzFast Octyl 4FF Prepacked column	1×1mL	Inquiry
CC02502313-02	EzFast Octyl 4FF Prepacked column	5×1mL	Inquiry
CC02502313-03	EzFast Octyl 4FF Prepacked column	1×5mL	Inquiry
CC02502313-04	EzFast Octyl 4FF Prepacked column	5×5mL	Inquiry
CC02502313-05	EzScreen Octyl 4FF Prepacked column	1×4.6mL	Inquiry
CC02502313-06	EzScreen Octyl 4FF Prepacked column	5×4.6mL	Inquiry
CC02502313-07	EzLoad 16/10 Octyl 4FF Prepacked column	1 pcs	Inquiry
CC02502313-08	EzLoad 26/10 Octyl 4FF Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Octyl Bestarose 4FF is obtained by coupling Octyl fat chains on a 4% agarose base matrix. The high physical and chemical stability of the agarose base matrix allows good pressure flow velocity performance, making it ideal for large-scale purification processes, especially in the capture or intermediate purification stages, where a high flow velocity separation process is required.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked agarose, 4%
Functional group	Octyl
Ligand concentration	~5μmol Ligand/mL resin
Particle size⁺	45~165um(the average particle size is 90μm)
Max. pressure	0.3MPa
Pressure flow velocity	≥150cm/h(0.1MPa BXK50/60, H=25cm, 20°C)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,1M HAc++,1mM HCl,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP), 3~13(working)
Operating temperature	Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C high pressure sterilization (20min)
Storage+++	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	90~150cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.