Diamond Butyl - Butyl HIC Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502315-01	EzFast Diamond Butyl Prepacked column	1×1mL	Inquiry
CC02502315-02	EzFast Diamond Butyl Prepacked column	5×1mL	Inquiry
CC02502315-03	EzFast Diamond Butyl Prepacked column	1×5mL	Inquiry
CC02502315-04	EzFast Diamond Butyl Prepacked column	5×5mL	Inquiry
CC02502315-05	EzScreen Diamond Butyl Prepacked column	1×4.6mL	Inquiry
CC02502315-06	EzScreen Diamond Butyl Prepacked column	5×4.6mL	Inquiry
CC02502315-07	EzLoad 16/10 Diamond Butyl Prepacked column	1 pcs	Inquiry
CC02502315-08	EzLoad 26/10Diamond Butyl Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Diamond Butyl is formed by coupling weakly hydrophobic aliphatic butyl groups on high-rigidity agarose matrix. It has low back pressure and fast flow velocity, which is suitable for large-scale purification of highly hydrophobic biomolecules.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked rigid agarose
Functional group	Butyl
Ligand concentration	~53μmol Ligand/mL resin
Dynamic binding capacity	~27mg BSA/mL packed resin
Particle size⁺	40~120μm
Max. pressure	0.5MPa
Pressure flow velocity	≥1200cm/h(0.5MPa BXK 100/500, H=20cm, 20°C)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,1M HAc++,6M GuHCl,30% isopropyl alcohol,70% ethanol
pH stability	2~14(CIP),3~13 (working)
Operating temperature	Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C autoclaving (20min)
Storage+++	2~30°C,20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~350cm/h

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH and 1M HAc only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer.