Diamond Butyl Mustang - Butyl HIC Chromatography Columns

Cat. No. Specification Unit Price Quantity
CC02502318-01 EzFast Butyl Mustang Prepacked column 1×1mL Inquiry
CC02502318-02 EzFast Butyl Mustang Prepacked column 5×1mL Inquiry
CC02502318-03 EzFast Butyl Mustang Prepacked column 1×5mL Inquiry
CC02502318-04 EzFast Butyl Mustang Prepacked column 5×5mL Inquiry
CC02502318-05 EzScreen Butyl Mustang Prepacked column 1×4.6mL Inquiry
CC02502318-06 EzScreen Butyl Mustang Prepacked column 5×4.6mL Inquiry
CC02502318-07 EzLoad 16/10 Butyl Mustang Prepacked column 1 pcs Inquiry
CC02502318-08 EzLoad 26/10 Butyl Mustang Prepacked column 1 pcs Inquiry

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Product Description
Product Description
Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Diamond Butyl Mustang is based on fine-grained high-rigidity agarose with weak hydrophobic aliphatic butyl groups. It has high resolution and low back pressure, and is suitable for the intermediate and fine purification stages of protein downstream purification.
Specifications

Appearance

White slurry

Matrix

Highly cross-linked rigid agarose

Functional group

Butyl

Average particle size

36~44μm

Hydrophobicity

The retention time of α - Chymotrypsinogen is 52-58min

Dynamic binding capacity

~37mg BSA/mL packed resin

Max. pressure

0.5MPa

Pressure flow velocity

≥450cm/h(0.5MPa BXK 100/500, H=20cm, 20°C)

Chemical stability

Stable in common aqueous buffers: 1M NaOH+,1M HAc+,6M GuHCl,30% isopropyl alcohol,70% ethanol

pH stability

2~14(CIP),3~13(working)

Operating temperature

Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C autoclaving (20min)

Storage++

2~30°C, 20% ethanol or 2% benzyl alcohol

Recommended flow velocity

90~180cm/h

+1M NaOH and 1M HAc only be used for cleaning.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.
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