Butyl-S Bestarose FF - Butyl HIC Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502317-01	EzFast Butyl-S FF Prepacked column	1×1mL	Inquiry
CC02502317-02	EzFast Butyl-S FF Prepacked column	5×1mL	Inquiry
CC02502317-03	EzFast Butyl-S FF Prepacked column	1×5mL	Inquiry
CC02502317-04	EzFast Butyl-S FF Prepacked column	5×5mL	Inquiry
CC02502317-05	EzScreen Butyl-S FF Prepacked column	1×4.6mL	Inquiry
CC02502317-06	EzScreen Butyl-S FF Prepacked column	5×4.6mL	Inquiry
CC02502317-07	EzLoad 16/10 Butyl-S FF Prepacked column	1 pcs	Inquiry
CC02502317-08	EzLoad 26/10 Butyl-S FF Prepacked column	1 pcs	Inquiry

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Product Description

Product Description

Hydrophobic interaction chromatography (HIC) is a chromatographic method that uses the interaction between the hydrophobic groups carried by biological molecules and the hydrophobic ligands on the stationary phase to separate substances. Salt ions can destroy the hydration membrane on the surface of biomolecules and promote the binding between hydrophobic groups and ligands.
Butyl-S Bestarose FF is formed by coupling butyl chain on 6% agarose base matrix. The optimized ligand concentration is suitable for the separation and purification of biological molecules with strong hydrophobicity.

Specifications

Appearance	White slurry
Matrix	Highly cross-linked agarose, 6%
Functional group	Butylthio
Ligand concentration	~ 10μmol Ligand/mL resin
Particle size⁺	45~165μm(the average particle size is 90μm)
Max. pressure	0.3MPa
Pressure flow velocity	250~400cm/h(0.1MPa BXK50/60, H =25cm, 20°C)
Chemical stability	Stable in common aqueous buffers: 20% ethanol ,6M GuHCl,0.5% SDS,8M Urea
pH stability	2~14(CIP),3~13(working)
Operating temperature	Operating temperature: 2~40°C, do not freeze, Can tolerate 121°C autoclaving (20min)
Storage++	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	150~300cm/h

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ 2% benzyl alcohol is only used for international transport or special requirements from customer.