DEAE Bestarose HP - Weak Anion Exchange Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502279-01	EzFast DEAE HP Prepacked column	1×1mL	Inquiry
CC02502279-02	EzFast DEAE HP Prepacked column	5×1mL	Inquiry
CC02502279-03	EzFast DEAE HP Prepacked column	1×5mL	Inquiry
CC02502279-04	EzFast DEAE HP Prepacked column	5×5mL	Inquiry
CC02502279-05	EzScreen DEAE HP Prepacked column	1×4.6mL	Inquiry
CC02502279-06	EzScreen DEAE HP Prepacked column	5×4.6mL	Inquiry
CC02502279-07	EzLoad 16/10 DEAE HP Prepacked column	1 pcs	Inquiry
CC02502279-08	EzLoad 26/10 DEAE HP Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determining the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
DEAE Bestarose HP (high performance) is a weak cation exchange resin formed by coupling diethylaminoethyl functional groups onto high flow velocity agarose matrix.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Functional Group	Diethylaminoethyl
Particle size+	24~44μm (the average particle size is 34μm)
Ionic capacity	110-160μmol/ mL packed resin
Max. pressure	0.3MPa
Pressure flow velocity	≥200cm/h (0.1MPa, BXK50/30, H=15cm, 25°C)
Working pH range	2~9
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,6M GuHCl,8M Urea, 70% ethanol ,30% isopropyl alcohol
pH Stability	2~14(CIP), 2~12 (Working)
Operating temperature	Operating temperature:2~40°C, do not freeze.
Storage+++	2~30°C, 20% ethanol or 2% benzyl alcohol
Recommended flow velocity	60~150cm/h
Avoid	Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20°C) to pH <4.

+Particle size is normally distributed, and particles within this range account for more than 75% of the total.
++1M NaOH only be used for cleaning.
+++ 2% benzyl alcohol is only used for international transport or special requirements from customer