DEAE Bestarose FF - Weak Anion Exchange Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502281-01	EzFast DEAE FF Prepacked column	1×1mL	Inquiry
CC02502281-02	EzFast DEAE FF Prepacked column	5×1mL	Inquiry
CC02502281-03	EzFast DEAE FF Prepacked column	1×5mL	Inquiry
CC02502281-04	EzFast DEAE FF Prepacked column	5×5mL	Inquiry
CC02502281-05	EzScreen DEAE FF Prepacked column	1×4.6mL	Inquiry
CC02502281-06	EzScreen DEAE FF Prepacked column	5×4.6mL	Inquiry
CC02502281-07	EzLoad 16/10 DEAE FF Prepacked column	1 pcs	Inquiry
CC02502281-08	EzLoad 26/10 DEAE FF Prepacked column	1 pcs	Inquiry
CC02502281-09	EzLoad 16/10 DEAE FF（Double stigma head） Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Ion exchange (IEX) chromatography is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability.
(2) Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
DEAE Bestarose FF (Fast Flow) is a weak anion exchange resin formed by coupling diethylaminoethyl to high-flow agarose microspheres.

Specifications

Appearance	White slurry
Matrix	Cross-linked agarose, 6%
Functional Group	Diethylaminoethyl
Particle size+	45~165μm
Ionic capacity	110-160μmol /mL packed resin
Max. pressure	0.3MPa
Pressure flow velocity++	≥300cm/h（0.1MPa ,BXK50/30,H=15cm ,20°C,0.1M NaCl）
Chemical stability	Stable in common aqueous buffers: 1M NaOH+++,1M HAC+++, 6M GuHCl,8M Urea, 70% ethanol,30% Isopropyl alcohol, 20% ethanol, 2%Benzyl alcohol
pH stability	2~14(CIP), 2~12 (Working)
Operating temperature	Operating temperature:2~40°C, do not freeze
Storage++++	2~30°C, 20%ethanol or 2% benzyl alcohol
Recommend flow velocity	60~300cm/h
Avoid	Avoid contact with oxidizing agents, cationic detergents. Avoid long time exposures (1 week, 20°C) to pH <4.

+Particle size is normally distributed, and particles within this range account for more than 95% of the total.
++ The pressure flow velocity describes the relationship between pressure and flow under the set circumstances. The pressure given shall not be taken as the maximum pressure of the resin.
+++1M NaOH and 1M HAc only be used for cleaning.
++++2% benzyl alcohol is only used for international transport or special requirements from customer.