Diamond DEAE - Weak Anion Exchange Chromatography Columns

Cat. No.	Specification	Unit	Price
CC02502280-01	EzFast Diamond DEAE Prepacked column	1×1mL	Inquiry
CC02502280-02	EzFast Diamond DEAE Prepacked column	5×1mL	Inquiry
CC02502280-03	EzFast Diamond DEAE Prepacked column	1×5mL	Inquiry
CC02502280-04	EzFast Diamond DEAE Prepacked column	5×5mL	Inquiry
CC02502280-05	EzScreen Diamond DEAE Prepacked column	1×4.6mL	Inquiry
CC02502280-06	EzScreen Diamond DEAE Prepacked column	5×4.6mL	Inquiry
CC02502280-07	EzLoad 16/10 Diamond DEAE Prepacked column	1 pcs	Inquiry
CC02502280-08	EzLoad 26/10 Diamond DEAE Prepacked column	1 pcs	Inquiry

Product Description

Product Description

Ion exchange chromatography (IEX) is a very effective method for the separation and purification of biomolecule. The method mainly relies on the interaction between positive and negative charges, and uses the charge properties and differences of different biological molecules under specific conditions to separate them. It has the characteristics of high load, good resolution, controllable condition and easy scale-up. It has been widely used in medicine, chemical industry, metallurgy, food and other fields.
IEX resin is composed of three parts:
(1) Cross-linked agarose matrix, that has the characteristics of porous, hydrophilic and good chemical stability, Traditional agarose molecules are modified to have better mechanical properties, which is called high rigid agarose, or Diamond base matrix.
(2)Functional groups fixed to the matrix, which are electrically charged groups, determine the properties of the IEX resin.
(3) An ion (called an equilibrium ion) that has an opposite charge to the functional group and can be reversibly bound to the functional group.
Diamond DEAE is a weak anion exchange resin formed by coupling diethylaminoethyl to high-rigidity agarose microspheres.

Specifications

Appearance	White slurry, can be layered
Matrix	Highly-rigidity agarose with long dextran chain
Functional group	Diethylaminoethyl
Average particle size⁺	~90μm
Ionic capacity	290~350μmol Cl^-/mL packed resin
Dynamic binding capacity	> 90mg ovalbumin /mL packed resin
Max. pressure	0.5MPa
Pressure flow velocity	≥1500cm/h(0.5MPa ,BXK 100/500 ,H=20cm, 20°C)
pH stability	2~9(working),2~14(CIP)
Chemical stability	Stable in common aqueous buffers: 1M NaOH++,1M HAC++, 6M GuHCl,8M Urea, 30% isopropyl alcohol, 70% ethanol Avoid contact with oxidizing agents, cationic detergents.
Temperature tolerance	Working temperature:2~40°C, Can't freeze.
Storage+++	2~30°C,20%ethanol or 2% benzyl alcohol
Recommended flow velocity	90~500cm/h

+ The average particle size is the cumulative resin particle size of the filler volume distribution.
++1M NaOH and 1M HAc only be used for cleaning.
+++2% benzyl alcohol is only used for international transport or special requirements from customer.