TOR Straight Shaft Stirred Bioreactor (5L-1000L)

TOR single-use & direct-axis stirred serial bioreactor, an appliance designed based on the mixing principle for traditional bioreactor, is featured with superior performances involving both mixing culture effect of traditional stirring and single-use process unit free from cleaning and sterilization verification, and it is a highly efficient, easy-to-use and powerful cell culture platform made up of hardware, software and consumables, achieving the best cell growth, cell vitality and metabolic process, and covering the scale ranging from 5L to 2000L.

Bioreactor Tank Design
Stir
• Using a low shear force downward pressure stirring paddle to form an axial flow,
• Fully mixing the materials in the tank and keeping the cells in the culture system under the suspension status to enable the sufficient contact of cells and nutrient substances;
• The low shear force stirring paddle disperses the bottom bubbles and evenly distributes them into the culture medium,
• Improving the mass transfer efficiency between gas and liquid to meet the needs of cell culture.

Heating and Cooling
• Connect the PT1000 sensor with a doublelayer wall jacket and associate it with a TCU temperature control unit to ensure efficient heating, cooling, and uniformity of solution temperature. Provide temperature protection, over temperature alarm, and automatic stop of heating due to overheating.

Control Cabinet Design
• Control system adopts Siemens PLC, which works with sensor and hardware meeting cell culture requirements to form PID closed-loop control;
• 15" colour touch screen is used for local operation, flow and data display, dual data backup and has power-off protection function.
• The software system complies with the standards for computer system from development to GMP production phase and relevant requirements of GAMP5 and 21 CFR Part 11.

Do Dissolved Oxygen
The precisely positioned bottom sparging aeration offers both ring and micro modes, and the DO sensor can be selected with traditional optical electrodes or disposable fiber optic electrodes for online monitoring and calibration, and flexible switching according to process needs.
• Automatic mode: real-time control of DO value in the process, DO sensor is with Air, N2, O2 mass flow controller Close vent and open relief valve, to achieve closed-loop control to form a cumulative amount, the system records the realtime flow of gas and counts the cumulative value.
• Manual mode: Air, N2 and O2 can be controlled independently in the HMI, and the system can record and count the real-time traffic and cumulative value of Air, N2 and O2.

Pressure Protection
If the pressure sensor exceeds the set value, the system automatically Close vent to ensure that the cell culture process maintains the proper pressure and avoids damage to the TOR reaction bag.

pH Monitoring
pH sensors can be selected with traditional optical sensor or disposable optical sensor for online monitoring and calibration, and pH control in the process in conjunction with acid-base pumps.
• Automatic mode: pH sensor is associated with acid-base pump and CO mass flow controller to achieve closed-loop control and record acid-base cumulative flow.
• Manual mode: Acid, alkali pump, CO mass flow controller can be controlled independently in the HMI.

Peristaltic Pumps and Weighing
• Refill pump: Replenish the liquid into the reactor
• Automatic mode: The rehydration pump can be linked to the weighing for closed-loop control. Automatic stop when the set weight is reached, the system records and counts the cumulative value of the rehydration pump
• Manual mode: The manual state can be independently controlled in the man-machine interface, and the system records and counts the cumulative value of the rehydration pump.
• Timing mode: Set the flow rate and time according to the total amount of rehydration.

• Hardware, software and consumables form an efficient, easy-touseand powerful cell culture platform that supports cell growth,cell viability and metabolic process
• Axial flow mixing and excellent material transfer effect
• Top quality accessories, stable run of system
• Modular design, supporting customization
• Three-level authority, audit trail, complete electronic data reporting, easy transfer and storage
• The touch screen software control function is rich, simple and intuitive, and the feedback system logic is clear
• Coarse bubble and micro bubble combining top overlay aeration and disposable reaction bag bottom sparging aeration design
• Complete FAT, SAT, IQ/OQ files and services

• Suspension cell culture;
• Adherent cell microcarrier culture;
• Process development;
• Process scale-up;
• Pilot test
• Inoculum amplification;
• GMP production.